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Rabbit anti-rat IgG, polyclonal antibody (HRP conjugate)

 
ADI-SAB-200-J 1 ml 133.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Purified rat IgG .
 
Species reactivity:Rat
 
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500)
Western Blot (1:5,000-100,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Quality Control:The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.
 
Formulation:Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/mL BSA (IgG-free, protease-free), and 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.
 

Product Literature References

Analysis of mucosal stress response in acid-induced esophagitis in opossum: W.G. Paterson, et al. ; Dig. Dis. Sci. 47, 1873 (2002), Application(s): WB using opossum samples, Abstract;
Nitric oxide (NO) induces nitration of protein kinase Cepsilon (PKC epsilon), facilitating PKC epsilon translocation via enhanced PKC epsilon -RACK2 interactions: a novel mechanism of no-triggered activation of PKC epsilon: P. Ping, et al. ; J. Biol. Chem. 277, 15021 (2002), Application(s): WB using human & rabbit samples, Abstract;

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