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ISH Virus Detection

Detect and Identify HPV & Viral DNA In Situ

  • PATHO-GENE® HPV Detection Assays and Specific Probes
  • BIO-PROBE® Virus Detection Assays


  • Cell Cycle Analysis
  • Cell Death
  • Cell Lineage Tracing
  • Cell Senescence
  • Cell Viability

Complete, Easy Workflow For ISH Detection

Pioneers in the Development of HPV Detection Systems

As a long time contributor to the HPV market, Enzo developed the first non-radioactive HPV probes for in situ hybridization detection in 1986. Today, Enzo continues to contribute to the field of HPV research. By utilizing extensive knowledge of gene expression analysis and a proficiency in novel dye chemistry for cell analysis, we have developed a new molecular platform, FLOWSCRIPT® HPV E6/E7 assay. As a pioneer in the development of HPV detection systems, Enzo continues to bring forward innovative technologies that provide clinically relevant information to researchers studying human papillomavirus.

Featured Literature

Human Papillomavirus - Clear Results, Innovative Solutions for HPV Detection

Traditional DNA ISH

PATHO-GENE® HPV Detection Assays and Specific Probes

HPV Probes and typing assays for sensitive detection of pathogen-expressed genes from fresh or FFPE tissue sections.

  • Specific, sensitive probes detect a broad spectrum of HPV types ranging from low to high risk
  • Complete assays provide comprehensive solution for identifying HPV DNA
  • Amenable to high-throughput analysis on automated staining systems

CIN/condylomata biopsy specimen infected with HPV type 16 DNA. The HPV 16/18 Probe Reagent exhibited strong nuclear staining. Tissue sections were developed with (A) HRP-AEC (100X) and (B) HRP-DAB (400X) and counter-stained with hematoxylin.

BIO-PROBE® Virus Detection Assays

Virus Detection Assays for use in a variety of hybridization techniques, including in situ hybridization at low concentrations (50-100 ng/mL). Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes.

“ Results with the Enzo probe were so good that we did not continue trying to optimize with a different probe.”

– Momin T. Siddiqui, MD, Department of Pathology and Laboratory Medicine, Emory University Hospital

(excerpt from Appl Immunohistochem Mol Morphol 2014; 22: 619–622)



  1. Brown DR, et. al. Neutralization of human papillomavirus type 11 (HPV-11) by serum from women vaccinated with yeast-derived HPV-11 L1 virus-like particles: correlation with competitive radioimmunoassay titer.J Infect Dis. 2001 Nov 1;184(9):1183-6.
  2. Lajer CB, et. al. Different miRNA signatures of oral and pharyngeal squamous cell carcinomas: a prospective translational study. Br J Cancer. 2011 Mar 1;104(5):830-40.
  3. Nuovo GJ, et. al. Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection. Diagn Mol Pathol. 2010 Sep;19(3):135-43.


Multiplex Analysis of Genotypic and Phenotypic Markers via Flow Cytometry


The assay employs a novel in situ hybridization technique utilizing a cocktail of probes specific to multiple sites within the E6 and E7 genes to ensure the detection of mRNA transcripts associated with the most prevalent high risk HPV genotypes.

  • Homogeneous system omits washing following hybridization and thereby minimizes both leakage and signal degradation resulting in:
    • More reliable, consistent results
    • Fewer steps and improved ease-of-use
  • Optimized design includes external controls allowing for consistency in assay performance
  • Adaptable to high throughput platforms for rapid sample analysis
  • Minimal sample volume input saves precious samples for additional analyses

Results in Less Than 3 Hours

Visualize Cellular Responses to Viral Infection

Comprehensive Solutions for Apoptosis

Apoptosis (PCD Type 1) is the most-well characterized, being recognized as a critical regulator of development, immunity, as well as organ and tissue homeostasis. Apoptotic cells die in a controlled fashion in response to a variety of extrinsic or intrinsic signals (e.g., activation of TNF receptors, DNA damage, mitochondrial pathways). With over 2000 products for the analysis of cell death, Enzo enables detection of phenotypic hallmarks of apoptotic cell death.

Functional Responses of Infected Cells

In addition to morphology, subcellular organelle functional response is a useful parameter for assessing cellular physiology. This includes phenomena such as nuclear DNA replication during cell cycle progression, DNA Damage, cellular senescence and phospholipid redistribution into the plasma membranes of the daughter cells upon proliferation, all of which can be modulated by various chemical, biological and environmental agents.

Cell Cycle Analysis
Cell Lineage Tracing
Cell Senescence
Cell Viability


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