Product Specification
| Alternative Name: | Glyceraldehyde-3-phosphate dehydrogenase |
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| Clone: | GA1R |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Full-length recombinant GAPDH. |
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| UniProt ID: | P04406 |
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| Species reactivity: | Human, Mouse, Rat
Chicken, E. coli, Hamster, Insect, Rabbit, Yeast
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| Specificity: | Recognizes GAPDH in multiple species, including human, mouse, rat, bacteria, chicken, hamster, insect, rabbit and yeast. |
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| Applications: | WB
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| Purity Detail: | Purified |
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| Formulation: | Liquid. In PBS containing a proprietary stabilizer and no preservative. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in Western blot analysis. Additionally, the antigen is conserved in most eukaryotes. |
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Fig. 1. Western Blot with GAPDH, mAb (GA1R) (HRP conjugate) (ENZ-ABS276-0050). Western blot analysis of GAPDH was performed by loading 50ug of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GAPDH monoclonal antibody (ENZ-ABS276-0050) at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed.
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Product Literature References
Analysis of oncogenic activities of protein kinase D1 in head and neck squamous cell carcinoma: L. Zhang, et al.; BMC Cancer
18, 1107 (2018),
Abstract;
Full Text
Amblyomma maculatum SECIS binding protein 2 and putative selenoprotein P are indispensable for pathogen replication and tick fecundity: K. Budachetri, et al.; Insect Biochem. Mol. Biol.
88, 37 (2017),
Abstract;
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