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GAPDH monoclonal antibody (GA1R) (HRP conjugate)

ENZ-ABS276-0050 50 µl 192.00 USD
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Product Specification

Alternative Name:Glyceraldehyde-3-phosphate dehydrogenase
Immunogen:Full-length recombinant GAPDH.
UniProt ID:P04406
Species reactivity:Human, Mouse, Rat
Chicken, E. coli, Hamster, Insect, Rabbit, Yeast
Specificity:Recognizes GAPDH in multiple species, including human, mouse, rat, bacteria, chicken, hamster, insect, rabbit and yeast.
Purity Detail:Purified
Formulation:Liquid. In PBS with 0.07% Kathon.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in Western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
Regulatory Status:RUO - Research Use Only
GAPDH monoclonal antibody (GA1R) (HRP conjugate) Western blot
Fig. 1. Western Blot with GAPDH, mAb (GA1R) (HRP conjugate) (ENZ-ABS276-0050). Western blot analysis of GAPDH was performed by loading 50ug of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GAPDH monoclonal antibody (ENZ-ABS276-0050) at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed.
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GAPDH monoclonal antibody (GA1R) (HRP conjugate) Western blot

Product Literature References

Analysis of oncogenic activities of protein kinase D1 in head and neck squamous cell carcinoma: L. Zhang, et al.; BMC Cancer 18, 1107 (2018), Abstract; Full Text
Amblyomma maculatum SECIS binding protein 2 and putative selenoprotein P are indispensable for pathogen replication and tick fecundity: K. Budachetri, et al.; Insect Biochem. Mol. Biol. 88, 37 (2017), Abstract;

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