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United States 

Caspase-4 (human), (recombinant) (active)

ALX-201-093A-U025 25 U 317.00 USD
ALX-201-093A-U100 100 U 612.00 USD
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Replaces Prod. #: ALX-201-093

Product Details

Alternative Name:ICH-2, Protease TX, ICErel-II
Source:Produced in E. coli. Contains an N-terminal His-tag.
UniProt ID:P49662
Purity:≥95% (SDS-PAGE)
Specific Activity:~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Application Notes:Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
Reconstitution:Reconstitute with PBS containing 15% glycerol to 1U/µl.
Shipping:Dry Ice
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Scientific Background:Caspase-4 is a member of the caspase family of cysteine proteases. It exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA.
Regulatory Status:RUO - Research Use Only

Product Literature References

MDA-7/IL-24 potently induces apoptosis in human myeloid leukemia cells through a process regulated by ER stress: M. Rahmani, et al.; Mol. Pharmacol. 78, 1096 (2010), Abstract; Full Text
The pan-HDAC inhibitor vorinostat potentiates the activity of the proteasome inhibitor carfilzomib in human DLBCL cells in vitro and in vivo: G. Dasmahapatra, et al.; Blood 115, 4478 (2010), Abstract; Full Text