Product Details
| Alternative Name: | NPY1R |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 237-252 of human NPY1R (neuropeptide Y1 receptor). |
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| UniProt ID: | P25929 |
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| Species reactivity: | Human, Rat
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| Applications: | ICC, IHC, WB
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| Blocking Peptide: | Included: Neuropeptide Y1 Receptor Control Peptide (40µg). Supplied lyophilized.
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| Purity Detail: | Affinity purified IgG. |
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| Formulation: | Lyophilized from PBS, pH 7.4, 1% BSA and 0.05% sodium azide. |
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| Reconstitution: | To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. To lyophilized peptide, add 100µl of deionized water. |
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| Use/Stability: | Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The NPY receptors belong to the G protein-coupled receptor superfamily. Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase, and a number of ion channels. NPY1R is considered to be a postsynaptic receptor. In the CNS, NPY1Rs are concentrated in the cortical areas of the brain, cerebral cortex, olfactory nucleus, hippocampus, hypothalamic nuclei, and thalamic nuclei. In addition, this receptor has a bimodal role in the immune system, serving as a strong negative regulator on T cells, as well as an activator of antigen-presenting cell function. |
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| Technical Info/Product Notes: | PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Jurkat cells (lanes 1, 4); K562 cells (lanes 2, 5); RBL cells (lanes 3, 6); rat brain lysates (lanes 7, 8). Lanes 1, 2, 3, 7: Anti-NPY1R. Lanes 4, 5, 6, 8: Anti-NPY1R preincubated with the control peptide.
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Product Literature References
Gene therapy mediated seizure suppression in Genetic Generalised Epilepsy: Neuropeptide Y overexpression in a rat model: K.L. Powell, et al.; Neurobiol. Dis.
113, 23 (2018),
Abstract;
Neuropeptide Y receptor 1 is expressed by B and T lymphocytes and mast cells in infantile haemangiomas: E.M. Tan, et al.; Acta Paediatr.
106, 292 (2017),
Abstract;
