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MART-1 (human) clonal antibody (A19-P)

ALX-810-217-R100 100 µl 273.00 USD
ALX-810-217-L001 1 ml 910.00 USD
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Product Details

Alternative Name:Melan A, Melanoma antigen recognized by T cells 1
Immunogen:Synthetic peptide corresponding to a C-terminal sequence of human MART-1 (melanoma antigen recognized by T cells 1).
UniProt ID:Q16655
Species reactivity:Human
Applications:IHC (FS), IHC (PS)
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:100-1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide.
Use/Stability:Stable for at least 12 months after receipt when stored as recommended.
Handling:After opening, prepare aliquots and store at +4°C.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Technical Info/Product Notes:Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.
Protocol:IHC (paraffin sections) Protocol:
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each
2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each
3. Rinse in distilled water
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes
5. Wash in distilled water for 5 minutes
6. For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in microwave (600W) for 10 minutes
7. Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 15 minutes
8. Rinse in distilled water
9. Wash in 0.05M TRIS/HCl, pH 7.6, supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes
10. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100-200 for 1 hour in the closed wet chamber
11. Wash twice 5 minutes with buffer A
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB)
13. Wash twice 5 minutes with buffer A
14. Apply the chromogen (DAB), 10 minutes
15. Wash in water for 10 minutes
16. Stain in hematoxylin for 5 minutes
17. Wash in water for 10 minutes
18. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each
19. Wash the section in 2 changes of xylene for 2 minutes each
20. Mount the slide for observation

*Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0):
Citric acid (anhydrous) ---------- 1.92g
Distilled water --------------------- 1000ml
Mix to dissolve in 700ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at 4°C for longer storage.
Regulatory Status:RUO - Research Use Only
MART-1 (human) clonal antibody (A19-P) Immunohistochemistry
Figure: Formalin-fixed and paraffin-embedded human melanoma (4μm) stained with cAb to MART-1 (human) (Prod. No. ALX-810-217) shows positive cytoplasmic immunostaining of tumor cells.Kindly performed and provided by Katarína Poliaková, MD and Lubomír Straka, MD, Ph.D. from Clinical Pathology Presov, Ltd., Presov, Slovakia.
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MART-1 (human) clonal antibody (A19-P) Immunohistochemistry

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