Purified from concentrated hybridoma tissue culture supernatant.
Species reactivity:
Human
Applications:
WB
Application Notes:
Excellent for Western blot.
Formulation:
Liquid. In PBS containing PEG-sucrose and 0.02% sodium azide.
Handling:
Thaw at +37°C. Avoid freeze/thaw cycles.
Shipping:
Dry Ice
Short Term Storage:
+4°C
Long Term Storage:
-80°C
Regulatory Status:
RUO - Research Use Only
Figure 1: Equal numbers of Jurkat cells treated and untreated with IFNa compared with equal numbers of CV1-EBNA cells transfected with human TRAIL and a control expression plasmid (not encoding hTRAIL) were analyzed by using HS501 (Prod. No. ALX-804-300.Method: Jurkat cells were treated with IFNa [1000 IU/ml] for 8 hours (lane 1) or were left untreated (lane 2), and CV1-EBNA cells were transfected with pcDNA3 expression vector encoding human TRAIL (lane 3), or with human TRAIL-R1 (lane 4). Lysates of equal numbers of cells were analyzed by Western blot with anti-TRAIL HS501 (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by horseradish peroxidase-conjugated anti-mouse IgG1 antibody and developed by ECL.
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Figure 2: Equal numbers of CV1-EBNA cells transfected with control expression plasmids (negative for human TRAIL) were analyzed and compared to a positive control cell-lysate using HS501.Method: CV1-EBNA cells, either transfected with pcDNA3 expression vector encoding TRAIL-R1 to TRAIL-R4 (lanes 1-4), or with human TRAIL (lane 5) were analyzed with HS501 MAb against TRAIL (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by a horseradish peroxidase conjugated anti-mouse IgG1 antibody and developed by ECL.
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Product Literature References
Pediatric multicellular tumor spheroid models illustrate a therapeutic potential by combining BH3 mimetics with Natural Killer (NK) cell-based immunotherapy: V. Särchen, et al.; Cell Death Discov. 8, 11 (2022), Abstract;
TNF-Related Apoptosis-Inducing Ligand Mediates Tumoricidal Activity of Human Monocytes Stimulated by Newcastle Disease Virus: B. Washburn, et al.; J. Immunol. 170, 1814 (2003), Abstract;
