Product Details
| Alternative Name: | Uvomorulin, CD324, Epithelial cadherin, Cadherin-1 |
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| Clone: | ECCD-1 |
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| Host: | Rat |
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| Isotype: | IgG2b |
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| Immunogen: | Mouse teratocarcinoma cell line F9. |
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| UniProt ID: | P09803 |
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| Source: | Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of Wistar rat immunized with mouse teratocarcinoma cell line F9. Purified from serum-free cell culture supernatant. |
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| Species reactivity: | Mouse
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| Specificity: | Epitope is close to the N-terminus of E-cadherin. |
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| Crossreactivity: | Does not cross-react with human, bovine, chicken or dog E-cadherin. |
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| Applications: | FUNC
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| Recommended Dilutions/Conditions: | Functional Application (200µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Functional Application: inhibits E-cadherin-dependent cell-cell contact. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Lyophilized from affinity purified antibody, dissolved in 10mM PBS, pH 7.4, containing 1.0% BSA. (contains no preservatives) |
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| Reconstitution: | Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C. |
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| Use/Stability: | The stock solution should be stored in aliquotes at -20°C for 1 year or should be stored at +4°C for 6 months after adding 0.1% sodium azide. |
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| Handling: | Dilute only prior to immediate use. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Ca2+ should be contained in all the buffers to stabilize cadherin antigen. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA: A. Nagafuchi, et al.; Nature
329, 341 (1987),
Abstract;
Molecular nature of the calcium-dependent cell-cell adhesion system in mouse teratocarcinoma and embryonic cells studied with a monoclonal antibody: C. Yoshida-Noro, et al.; Dev. Biol.
101, 19 (1984),
Abstract;
