Product Details
| Alternative Name: | PARP-4, VPARP |
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| Clone: | p193-10 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Minor vault p193 protein (VPARP; PARP-4). |
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| UniProt ID: | Q9UKK3 |
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| Species reactivity: | Human
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| Specificity: | Recognizes epitope aa 506-510 (VALGK) of human minor vault p193 protein. |
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| Applications: | IHC (FS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry: Use 1:10-1:20 dilution for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. Apply the following pretreatment: 10 min in 20mM glycine in PBS (pH 7.5) and 10 min in 6N guanidine hydrochloride in 50mM TRIS-HCl (pH 7.5); Schroeijers et al., (2000).
Western Blot: Use 1:10-1:20 dilution (ECL)
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines: A.B. Schroeijers, et al.; Cancer Res.
60, 1104 (2000),
Abstract;
Full Text
