Product Details
| Alternative Name: | ADRA1B, AR α-1b |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of rat adrenergic receptor α1b. |
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| UniProt ID: | P15823 |
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| Species reactivity: | Rat
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| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: human, mouse, bovine, rhesus macaque, rabbit, wild boar, dog. |
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| Applications: | ELISA, IHC, WB
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| Application Notes: | Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~56kDa. Higher MW species also observed by Western blot which reflect post-translational modification of the receptor. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
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| Handling: | Keep on ice at all times. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Adrenergic receptors mediate the hormone and neurotransmitter functions of adrenaline and noradrenaline. Adrenoreceptors function in peripheral tissues and the central nervous system to regulate numerous vascular, metabolic, and neurological functions, and are the target for many therapeutically relevant drugs including those for the treatment of hypertension, asthma, benign prostatic hypertrophy, and depression. Adrenoreceptors are classified by three types (α1, α2, and β) and multiple subclasses based on pharmacological and molecular characteristics. The α1 family consists of three receptor subtypes (α1a, 1b, 1d) which primarily signal via Gq/11 alpha subunits to stimulate Phospholipase C and increase intracellular calcium. In contrast, the three α2 family receptor subtypes (α2a, 2b, 2c) signal via Gi/o alpha subunits to inhibit adenylyl cyclase activity, increase potassium transport, inhibit calcium conductance, and stimulate phospholipase A2. α1b adrenergic receptor mRNA expression in human tissues is highest in the spleen, kidney, and fetal brain. The α2a receptor is most highly expressed in human brain, spleen, and kidney, and is a species orthologue of the α2d receptor expressed similarly in rat, mouse, and cow. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by Proteimax. |
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Western blot analysis: MW marker (1) and 20µg rat brain extract (2) probed with Adrenergic Receptor (alpha 1b) pAb at 5.9µg/ml.
Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 μM concentrations of agonist (UK14304) and probed with Adrenergic Receptor (alpha 1b) pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4).
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Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
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