Product Details
| Alternative Name: | ADRB1, AR β-1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to a 1.2kDa peptide near the N-terminus of rat adrenergic receptor β1. |
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| UniProt ID: | P18090 |
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| Species reactivity: | Rat
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| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: human, mouse, guinea pig, wild boar, chimpanzee, dog, cat, rhesus macaque, bovine, opossum. |
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| Applications: | ELISA, WB
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| Application Notes: | Suitable for membrane-based ELISA.
Predicted MW of ~50kDa. Higher MW species (~75kDa) and intermediates also observed by Western blot which reflect post-translational modification and dimerization of the receptor. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
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| Handling: | Keep on ice at all times. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Adrenergic receptors mediate the hormone and neurotransmitter functions of adrenaline and noradrenaline. Adrenoreceptors function in peripheral tissues and the central nervous system to regulate numerous vascular, metabolic, and neurological functions, and are the target for many therapeutically relevant drugs including those for the treatment of hypertension, asthma, benign prostatic hypertrophy, and depression. Adrenoreceptors are classified by three types (α1, α2, and β) and multiple subclasses based on pharmacological and molecular characteristics. The three β adrenergic receptor subtypes (β 1, 2, 3) signal primarily via coupling to GS alpha subunits, stimulating production of cAMP and activation of protein kinase A to regulate opening of L-type Ca2+ channels. Expression of β1 receptors is highest in the heart and in adipose tissue, displaying relatively equal sensitivity to adrenaline and noradrenaline. The β 2 receptor mediates relaxation in vascular, uterine, and pulmonary smooth muscle, and shows greater sensitivity to noradrenaline compared to adrenaline. Agonist affinity to β2 adrenergic receptors is modulated by Zn2+ concentration, and may also trigger cGMP accumulation and protein kinase G activation via Gi/o alpha subunit coupling in some cell types. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by Proteimax. |
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Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1 μM concentrations of agonist (Isoproterenol) and probed with Adrenergic Receptor (β1) pAb (1:25 to 1:200 of a 0.1µg/μL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=5).
Western blot analysis: MW marker (1) and 20µg rat heart membrane extract (2) probed with Adrenergic Receptor (β1) pAb 2.0µg/ml.
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Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
Full Text
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