As a long time contributor to the HPV market, Enzo developed the first non-radioactive HPV probes for in situ hybridization detection in 1986. Today, Enzo continues to contribute to the field of HPV research. By utilizing extensive knowledge of gene expression analysis and a proficiency in novel dye chemistry for cell analysis, we have developed a new molecular platform, FLOWSCRIPT® HPV E6/E7 assay. As a pioneer in the development of HPV detection systems, Enzo continues to bring forward innovative technologies that provide clinically relevant information to researchers studying human papillomavirus.
Oncogene Expression
FLOWSCRIPT® HPV E6/E7 Assay
Cell Cycle Control
NUCLEAR-ID® Cell Cycle Kits
NUCLEOLAR-ID® Detection Kit
Cell Cycle Arrest | DNA Repair | Apoptosis
DNA Damage ELISA Kit
Comet SCGE Assay Kit
Annexin V Conjugates
GFP-CERTIFIED® Apoptosis/Necrosis Detection Kit
NUCLEAR-ID® Chromatin Condensation Detection Kits
Replicative Senescence
Cellular Senescence Activity Assay
Cellular Senescence Live Cell Analysis Assay
p21 and p53 ELISAs
In Situ Hybridization
BIO-PROBE® Labeling Systems
POLYVIEW® PLUS Detection Reagents
PATHO-GENE® & BIO-PROBE® Virus Detection Assays
HPV Probes and typing assays for sensitive detection of pathogen-expressed genes from fresh or FFPE tissue sections.
CIN/condylomata biopsy specimen infected with HPV type 16 DNA. The HPV 16/18 Probe Reagent exhibited strong nuclear staining. Tissue sections were developed with (A) HRP-AEC (100X) and (B) HRP-DAB (400X) and counter-stained with hematoxylin.
Virus Detection Assays for use in a variety of hybridization techniques, including in situ hybridization at low concentrations (50-100 ng/mL). Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes.
– Momin T. Siddiqui, MD, Department of Pathology and Laboratory
Medicine, Emory University Hospital
(excerpt from Appl Immunohistochem Mol Morphol 2014; 22: 619–622)
References:
The assay employs a novel in situ hybridization technique utilizing a cocktail of probes specific to multiple sites within the E6 and E7 genes to ensure the detection of mRNA transcripts associated with the most prevalent high risk HPV genotypes.
Apoptosis (PCD Type 1) is the most-well characterized, being recognized as a critical regulator of development, immunity, as well as organ and tissue homeostasis. Apoptotic cells die in a controlled fashion in response to a variety of extrinsic or intrinsic signals (e.g., activation of TNF receptors, DNA damage, mitochondrial pathways). With over 2000 products for the analysis of cell death, Enzo enables detection of phenotypic hallmarks of apoptotic cell death.
In addition to morphology, subcellular organelle functional response is a useful parameter for assessing cellular physiology. This includes phenomena such as nuclear DNA replication during cell cycle progression, DNA Damage, cellular senescence and phospholipid redistribution into the plasma membranes of the daughter cells upon proliferation, all of which can be modulated by various chemical, biological and environmental agents.
Cell Cycle Analysis
Cell Lineage Tracing
Cell Senescence
Cell Viability
