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Nick translation DNA labeling system

Nick translation  system for preparing FISH probes
 
ENZ-42910 50 Reactions 282.00 USD
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The Nick Translation DNA Labeling System provides a simple and efficient method for generating labeled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1kb for fluorescent in situ hybridization (FISH) applications. The Nick Translation DNA Labeling System can accommodate a wide range of biotin-labeled and fluorophore-labeled nucleotides. In addition to choice of label, the kit design allows the user to optimize incorporation and product size by adjusting the ratio of labeled-dUTP to dTTP and DNase I concentration respectively.
ENZ-42910 specific activity
Column purified labeled FISH probes demonstrate consistent incorporation and specific activity. The nick translation reaction enables rapid and consistent production of labeled DNA, accommodates a wide range of labeled nucleotides and is ideal for multiplexed applications.


ENZ-42910 Fish Probe
Labeling DNA probe for FISH analysis
ENZ-42910 Nick Translation
Blood Sample hybridized with FISH probes made with Nick translation DNA labeling system (Prod No. ENZ-42910) and Orange 552 dUTP (Prod No. ENZ-42842) or Green 496 dUTP (Prod No. ENZ-42831). [telomere 7p (spectrum orange, Orange 552 dUTP) RP11-452K21 en 7q (spectrum green, Green 496 dUTP)] Analysis with an epifluorescence microscope using DAPI, FITC and TRITC single band filters. Courtesy of GH Necker-Enfants Malades (Paris, FR).
ENZ-42910 chromosomespread
Composite fluorescent image using the Nick Translation DNA Labeling System and BAC Target DNA labeled with Enzo’s Green 496 dUTP (ENZ-42831) and Centromere BAC Probe labeled with Enzo’s Orange 552 dUTP (ENZ-42842). Metaphase chromosome spreads were counterstained with DAPI.
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ENZ-42910 specific activity ENZ-42910 Fish Probe ENZ-42910 Nick Translation ENZ-42910 chromosomespread

Product Specification

Quality Control:Performance Testing: A sample kit from each lot of Nick Translation DNA Labeling System is used to label 1.0µg BAC DNA with Orange 552 dUTP (Prod. No. ENZ-42842) and hybridized to metaphase spreads using the protocols described in the user manual.
Minimum Specifications:
- Electrophoresis of the reaction on a non-denaturing agrose gel must yield a distinct smear between 50 to 500 base pairs.
- An average of 100 picomoles of labeled nucleotide must be incorporated per μg of sample DNA as described by the method in the user manual.
- A fluorescent labeled locus-specific FISH probe generated from 1 µg BAC DNA must demonstrate chromosome specific hybridization confirmed by a labeled CEN probe.
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:DNase I 100 µL
dTTP 180 µL
DNA Polymerase I 250 µL
dNTP Mix 250 µL
Reaction Buffer 250 µL
Stop Buffer 250 µL
DNase I Dilution Buffer 1 mL 
Nuclease-free Water 10 mL
 

Product Literature References

Modeling genomic imprinting disorders using induced pluripotent stem cells: S.J. Chamberlain, et al.; Methods Mol. Biol. 1353, 45 (2016), Abstract;
MAR-Mediated Dystrophin Expression in Mesoangioblasts for Duchenne Muscular Dystrophy Cell Therapy: R.W. van Zwieten, et al.; Mol. Biol. 4, 2168 (2015), Application(s): Fluorescence in situ hybridization, Full Text
Imprinted expression of UBE3A in non-neuronal cells from a Prader-Willi syndrome patient with an atypical deletion: K. Martins-Taylor, et al.; Hum. Mol. Genet. 23, 2364 (2014), Application(s): Labeling DNA for FISH, Abstract; Full Text
Altered expression of MGMT in high-grade gliomas results from the combined effect of epigenetic and genetic aberrations: J. Ramalho-Carvalho, et al.; PLoS One 8, e58206 (2013), Application(s): Labeling DNA for FISH, Abstract; Full Text
Common fusion transcripts identified in colorectal cancer cell lines by high-throughput RNA sequencing: T. Nome, et al.; Transl. Oncol. 6, 546 (2013), Application(s): Labeling DNA for FISH, Abstract; Full Text

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