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Nick translation DNA labeling system

 
ENZ-42910 50 Reactions 270.00 USD
 
The Nick Translation DNA Labeling System provides a simple and efficient method for generating labeled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1kb for fluorescent in situ hybridization (FISH) applications. The Nick Translation DNA Labeling System can accommodate a wide range of biotin-labeled and fluorophore-labeled nucleotides. In addition to choice of label, the kit design allows the user to optimize incorporation and product size by adjusting the ratio of labeled-dUTP to dTTP and DNase I concentration respectively.

Product Specification

Quality Control:Performance Testing: A sample kit from each lot of Nick Translation DNA Labeling System is used to label 1.0µg BAC DNA with Orange 552 dUTP (Prod. No. ENZ-42842) and hybridized to metaphase spreads using the protocols described in the user manual.
Minimum Specifications:
- Electrophoresis of the reaction on a non-denaturing agrose gel must yield a distinct smear between 50 to 500 base pairs.
- An average of 100 picomoles of labeled nucleotide must be incorporated per μg of sample DNA as described by the method in the user manual.
- A fluorescent labeled locus-specific FISH probe generated from 1 µg BAC DNA must demonstrate chromosome specific hybridization confirmed by a labeled CEN probe.
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
Long Term Storage:-20°C
 
Kit/Set Contains:100 µL, DNase I 180 µL, dTTP 250 µL, DNA Polymerase I 250 µL, dNTP Mix 250 µL, Reaction Buffer 250 µL, Stop Buffer 1 mL, DNase I Dilution Buffer 10 mL, Nuclease-free Water
 

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