Nick translation DNA labeling system



ENZ-42910	1 Kit	50 reactions	248.00 USD

The Nick Translation DNA Labeling System provides a simple and efficient method for generating labeled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1kb for fluorescent in situ hybridization (FISH) applications. The Nick Translation DNA Labeling System can accommodate a wide range of biotin-labeled and fluorophore-labeled nucleotides. In addition to choice of label, the kit design allows the user to optimize incorporation and product size by adjusting the ratio of labeled-dUTP to dTTP and DNase I concentration respectively.

Product Specification

Quality Control:	Performance Testing: A sample kit from each lot of Nick Translation DNA Labeling System is used to label 1.0 μg BAC DNA with Orange 552 dUTP (Prod. No. ENZ-42842) and hybridizaed to metaphase spreads using the protocols described in the user manual. Minimum Specifications: - Electrophoresis of the reaction on a non-denaturing agrose gel must yield a distinct smear between 50 to 500 base pairs. - An average of 100 picomoles of labeled nucleotide must be incorporated per μg of sample DNA as described by the method in the user manual. - A fluorescent labeled locus-specific FISH probe generated from 1 μg BAC DNA must demonstrate chromosome specific hybridization confirmed by a labeled CEN probe.

Kit/Set Contains:	100 μL, DNase I 180 μL, dTTP 250 μL, DNA Polymerase I 250 μL, dNTP Mix 250 μL, Reaction Buffer 250 μL, Stop Buffer 1 mL, DNase I Dilution Buffer 10 mL, Nuclease-free Water

Long Term Storage:	-20°C

Use/Stability:	Stable for at least one year after receipt when stored as recommended.

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	For the Manual please click here.