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ULTRA SENSITIVE® Enhanced AP-NBT/BCIP in situ detection system

 
ENZ-32700 30 assays 220.00 USD
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The ULTRA SENSITIVE® Enhanced Alkaline Phosphatase-NBT/BCIP In Situ Detection System provides reagents and materials for the ultra-sensitive detection of biotin-labeled nucleic acid probes following in situ hybridization to fixed cells and tissues. The easy-to-use system consists of ready-to-use rabbit anti-Biotin, biotin-labeled goat anti-Rabbit IgG, alkaline phosphatase-streptavidin detection complex, NBT/BCIP reaction mixture and all buffers required for superior detection sensitivity.

Product Specification

Applications:Colorimetric detection
 
Use/Stability:Store the Red Counterstain at room temperature away from direct bright light. The shelf-life from the NBT/BCIP Reaction Mixture may be extended by storing the reagent at -20°C.
 
Handling:The shelf-life from the NBT/BCIP Reaction Mixture may be extended by storing the reagent at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:Post Hybridization Reagent, 15 ml (white) Buffered formamide
Antibody Blocking Buffer, 15 ml (green) Phosphate buffered saline containing blocking protein, stabilizer and preservative
Rabbit anti-Biotin, 15 ml (pink) Rabbit anti-Biotin in phosphate buffered saline with stabilizer and preservative
Bio-Goat anti-Rabbit IgG, 15 ml (purple) Biotin-labeled Goat anti-Rabbit IgG in phosphate buffered saline with stabilizer and preservative
AP Detection Reagent, 15 ml (orange) Streptavidin-biotinylated alkaline phosphatase complex in buffered saline with stabilizers and preservative
NBT/BCIP Reaction Mixture, 15 ml (blue) Ready-to-use reaction buffer containing nitroblue tetrazolium and bromochloroindolyl phosphate
Red Counterstain, 15 ml (red) Ready-to-use nuclear fast red counterstain
Wash Buffer Salts, 2 packets Each packet yields one liter of Antibody Wash Buffer (phosphate buffered saline, PBS)
SIGNASURE® Wash Buffer, 2 packets Each packet yields one liter of ready-to-use SIGNASURE® Wash Buffer
 
Technical Info/Product Notes:In nucleic acid assays, this product or its use are covered by one or more claims of Enzo patents, including, but not limited to the following: U.S. Patent No. 4,994,373; Canadian Patent No. 1,309,672; and patents pending.
 

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