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NGAL (human) monoclonal antibody (5G5)

 
BPD-HYB-211-01-02 200 µg 632.00 USD
 
BPD-HYB-211-01-1 1 mg 2,834.00 USD
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Product Specification

Alternative Name:Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
 
Clone:5G5
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Recombinant human NGAL in monomer and dimer forms.
 
UniProt ID:P80188
 
Species reactivity:Human
 
Applications:ELISA, IHC, WB
 
Application Notes:Detects a band of ~25kDa by Western blot.
 
Purity Detail:Protein A/G-affinity purified.
 
Formulation:Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Scientific Background:Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) belongs to the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, and occurs as 25-kDa glycosylated single protein chain monomers which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. The expression and release of NGAL from renal tubules is dramatically increased by ischemic or nephrotoxic injury.
 
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Figure 1: Immunohistochemical analysis of normal pancreas tissue using NGAL (human), mAb (5G5).
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Figure 2: Immunohistochemical analysis of chronic pancreatitis tissue using NGAL (human), mAb (5G5).
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Figure 3: Immunohistochemical analysis of tissue microarrays from human PaCa (MDACC) using NGAL (human), mAb (5G5).
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Product Literature References

Neutrophil gelatinase-associated lipocalin: a novel suppressor of invasion and angiogenesis in pancreatic cancer: Z. Tong, et al.; Cancer Res. 68, 6100 (2008), Abstract;
NGAL is an early predictive biomarker of contrast-induced nephropathy in children: R. Hirsch, et al.; Pediatr. Nephrol. 22, 2089 (2007), Abstract;
Plasma neutrophil gelatinase-associated lipocalin predicts acute kidney injury, morbidity and mortality after pediatric cardiac surgery: a prospective uncontrolled cohort study: C.L. Dent, et al.; Crit. Care 11, R127 (2007), Abstract;
Urine neutrophil gelatinase-associated lipocalin is an early marker of acute kidney injury in critically ill children: a prospective cohort study: M. Zappitelli, et al.; Crit. Care 11, R84 (2007), Abstract;
Association between increases in urinary neutrophil gelatinase-associated lipocalin and acute renal dysfunction after adult cardiac surgery: G. Wagener, et al.; Anesthesiology 105, 485 (2006), Abstract;
Urinary neutrophil gelatinase-associated lipocalcin in D+HUS: a novel marker of renal injury: H. Trachtman, et al.; Pediatr. Nephrol. 21, 989 (2006), Abstract;
Urinary neutrophil gelatinase-associated lipocalin as a biomarker of nephritis in childhood-onset systemic lupus erythematosus: H.I. Brunner, et al.; Arthritis Rheum. 54, 2577 (2006), Abstract;
Urine NGAL and IL-18 are predictive biomarkers for delayed graft function following kidney transplantation: C.R. Parikh, et al.; Am. J. Transplant. 6, 1639 (2006), Abstract;
Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery: J. Mishra, et al.; Lancet 365, 1231 (2005), Abstract;
The endocytic receptor megalin binds the iron transporting neutrophil-gelatinase-associated lipocalin with high affinity and mediates its cellular uptake: V. Hvidberg, et al.; FEBS Lett. 579, 773 (2005), Abstract;
Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils: L. Kjeldsen, et al.; J. Immunol. Methods 198, 155 (1996), Abstract;

General Literature References

Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), Abstract;

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