IL-1F5 (mouse), (recombinant)

[Interleukin-1 family member 5, IL-1δ, Interleukin-1δ]

Concentration:	1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Lonza).

MW:	~16kDa

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Source/Host:	Produced in E. coli (aa 2-156).

Reconstitution:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Shipping:	SHIPPED ON BLUE ICE

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	IL-1F5, F6, F8 and F9 are new members of the family of IL-1 (IL-1α/β, IL-18, IL-33). IL-1F6, IL-1F8 and IL-1F9 activate the NF-κB and MAPK pathways through the IL-Rrp2 and IL-1RAcP receptor complex leading to the production of IL-6 and IL-8. Agonistic activation of the IL-1F6, F8 and F9 is counterbalanced by the inhibitory activity of IL-1F5. These interleukins are mainly expressed in skin keratinocytes and internal epithelial tissues exposed to pathogenic bacteria suggesting a role in immune signalling and inflammation. IL-F5, F6 and F9 as well as the receptor IL-1Rrp2 are increased in psoriasis.

Background / Technical Information:	Swiss-Prot link Q9QYY1: Interleukin-1 Family Member 5 (mouse)