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IL-33 (rat), (recombinant)

ALX-522-125-C010 10 µg 383.00 USD
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Product Specification

Alternative Name:Interleukin-33, NF-HEV, IL-1F11
Source:Produced in E. coli. Untagged rat IL-33 (aa 109-264).
UniProt ID:Q66H70
Concentration:0.1mg/ml after reconstitution.
Formulation:Lyophilized. Contains PBS.
Purity:≥95% (SDS-PAGE)
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Cape Cod Associates).
Reconstitution:Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:IL-33 is a member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1RL1) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The human IL-33 protein is expressed with a prodomain (aa 1-111) which is cleaved by caspase-1 to produce the 18kDa mature protein (112-270).
ALX-522-125 SDS-PAGE
SDS-PAGE analysis: Lane 1: MW Marker; Lane 2: 1.0μg of purified IL-33 (rat), (recombinant).
ALX-522-125 WB
Western Blot analysis: Lane 1: MW Marker; Lane 2: 100ng of purified IL-33 (rat), (recombinant) protein probed with IL-33 pAb (Prod. no. ALX-210-933) at 0.5μg/ml.
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ALX-522-125 SDS-PAGE ALX-522-125 WB

Product Literature References

Nuclear Interleukin-33 Is Generally Expressed in Resting Endothelium but Rapidly Lost upon Angiogenic or Proinflammatory Activation: A.M. Küchler, et al.; Am. J. Pathol. 173, 1229 (2008), Abstract;

General Literature References

IL-33 and ST2 comprise a critical biomechanically induced and cardioprotective signaling system: S. Sanada, et al.; J. Clin. Invest. 117, 1538 (2007), Abstract; Full Text
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005), Abstract;

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