Product Details
Alternative Name: | Interleukin-33, NF-HEV, IL-1F11 |
|
MW: | ~18kDa |
|
Source: | Produced in E. coli. Untagged human IL-33 (aa 112-270). |
|
UniProt ID: | O95760 |
|
Concentration: | 0.1mg/ml after reconstitution. |
|
Formulation: | Lyophilized. Contains PBS. |
|
Purity: | ≥95% (SDS-PAGE) |
|
Purity Detail: | Purified by multi-step chromatography. |
|
Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.). |
|
Specificity: | Binds to human ST2. |
|
Reconstitution: | Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
|
Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
|
Scientific Background: | IL-33 is a member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1RL1) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The human IL-33 protein is expressed with a prodomain (aa 1-111) which is cleaved by caspase-1 to produce the 18kDa mature protein (112-270). Historical data has shown that IL-33 activates human ST2-dependent NF-κB pathway, but it does not activate mouse ST2-dependent NF-κB. |
|
Regulatory Status: | RUO - Research Use Only |
|
SDS-PAGE Analysis. Lane 1: MWM; Lane 2: 1.0 µg of purified IL-33 (human), (recombinant).
Please mouse over
Product Literature References
Interleukin-33 regulates tissue remodelling and inhibits angiogenesis in the eye: S. Theodoropoulou, et al.; J. Pathol.
241, 45 (2017),
Abstract;
Full Text
Human basophils and eosinophils are the direct target leukocytes of the novel IL-1-family member IL-33: T. Pecaric-Petkovic et al.; Blood
113, 1526 (2009),
Abstract;
IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo: V. Carriere et al.; PNAS
104, 282 (2007),
Abstract;
General Literature References
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity
23, 479 (2005),
Abstract;
Related Products