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IL-33 polyclonal antibody (AT110)

ALX-210-933-C100 100 µg 402.00 USD
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Product Details

Alternative Name:Interleukin-33, NF-HEV, IL-1F11
Immunogen:Recombinant human soluble IL-33 (interleukin-33) (aa 112-270) (Prod. No. ALX-522-098).
UniProt ID:O95760
Gene/Protein Identifier:90865 (Entrez GeneID), 608678 (OMIM)
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes pro-IL-33 and mature IL-33.
Applications:ELISA, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Prod. No. ALX-522-098; Prod. No. ALX-522-101
Purity Detail:Protein A-affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
IL-33 polyclonal antibody (AT110) Western blot
Figure: Endogenous IL-33 is detected in the supernatant of THP1 using PAb to IL-33 (AT110) (Prod. No. ALX-210-933). Method: THP-1 cells were treated with PBS (lane 1), monosodium urate (MSU) (Prod. No. ALX-400-047) (lane 2) or peptidoglycan (PGN) (lane 3). The supernatants were collected, precipitated and loaded in a 15% SDS-gel. Western blot was performed using PAb to IL-33 (AT110) or a PAb to IL-1ß (cell signalling)."
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IL-33 polyclonal antibody (AT110) Western blot

Product Literature References

Unveiling IL-33/ST2 Pathway Unbalance in Cardiac Remodeling Due to Obesity in Zucker Fatty Rats: C. Sitzia, et al.; Int. J. Mol. Sci. 24, 1991 (2023), Abstract;
Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer’s disease: J. Chapuis, et al.; Mol. Psychiatry 14, 1004 (2009), Abstract;
Nuclear Interleukin-33 Is Generally Expressed in Resting Endothelium but Rapidly Lost upon Angiogenic or Proinflammatory Activation: A.M. Küchler, et al.; Am. J. Pathol. 173, 1229 (2008), Abstract;
IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo : V. Carriere et al.; PNAS 104, 282 (2007), Abstract;

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