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IL-33 (mouse), (recombinant)

ALX-522-101-C010 10 µg 364.00 USD
ALX-522-101-3010 3x10 µg SuperPack 741.00 USD
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Product Details

Alternative Name:Interleukin-33, NF-HEV, IL-1F11
Source:Produced in E. coli. Untagged mouse IL-33 (aa 109-266).
UniProt ID:Q8BVZ5
Concentration:0.1mg/ml after reconstitution.
Formulation:Lyophilized. Contains PBS.
Purity:≥95% (SDS-PAGE)
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
Specificity:Binds to mouse and human ST2.
Reconstitution:Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:IL-33 is a member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1RL1) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The mouse IL-33 protein is expressed with a prodomain (aa 1-108) which is cleaved to produce the 18kDa mature protein (109-266). Historical data has shown that IL-33 activates mouse and human ST2-dependent NF-κB pathway and induces IL-6 production in P815 mastocytoma cells and in bone marrow derived mast cells.
Regulatory Status:RUO - Research Use Only
IL-33 (mouse), (recombinant) SDS-PAGE
SDS-PAGE analysis: Lane 1: MW Marker; Lane 2: 1.0ug of purified IL-33 (mouse), (recombinant).
IL-33 (mouse), (recombinant) Western blot
Western Blot analysis: Lane 1: MW Marker; Lane 2: 100ng of purified IL-33 (mouse), (recombinant) protein probed with IL-33 mAb (Prod. no. ALX-804-840PF-C100) at 0.5ug/ml.
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IL-33 (mouse), (recombinant) SDS-PAGE IL-33 (mouse), (recombinant) Western blot

Product Literature References

Treatment with interleukin-33 is non-toxic and protects retinal pigment epithelium in an ageing model of outer retinal degeneration: A.J. Clare, et al.; J. Cell. Mol. Med. 24, 13456 (2020), Abstract; Full Text
Endotoxin tolerance in mast cells, its consequences for IgE-mediated signalling, and the effects of BCL3 deficiency: M. Poplutz, et al.; Sci. Rep. 7, 4534 (2017), Abstract; Full Text
Interleukin-33 regulates tissue remodelling and inhibits angiogenesis in the eye: S. Theodoropoulou, et al.; J. Pathol. 241, 45 (2017), Abstract; Full Text
IL-33 Drives Eosinophil Infiltration and Pathogenic Type 2 Helper T-Cell Immune Responses Leading to Chronic Experimental Ileitis: C. De Salvo, et al.; Am. J. Pathol. 186, 885 (2016), Application(s): Experimental in Vivo Studies, Abstract;
Interleukin-33 promotes the proliferation of mouse mast cells through ST2/MyD88 and p38 MAPK-dependent and Kit-independent pathways: R. Saluja, et al.; J. Biol. Regul. Homeost. Agents. 28, 575 (2014), Abstract;
The novel cytokine interleukin-33 activates acinar cell proinflammatory pathways and induces acute pancreatic inflammation in mice: D. Kempuraj, et al.; PLoS One 8, e56866 (2013), Abstract; Full Text
IL-1 receptor accessory protein is essential for IL-33-induced activation of T lymphocytes and mast cells: S. Ali, et al.; PNAS 104, 18660 (2007), Abstract;

General Literature References

IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005), Abstract;

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