Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

TRAIL-R2 (mouse):Fc (human), (recombinant)

 
ALX-522-067-C050 50 µg 419.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:DR5, TNFRSF 10B, KILLER, CD262
 
MW:~62kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of mouse TRAIL-R2 (DR5) (aa 1-166) is fused to the Fc portion of human IgG1.
 
UniProt ID:P50592
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Specificity:Binds mouse and less potently human soluble TRAIL.
 
Applications:Flow Cytometry
 
Application Notes:Flow Cytometry: Cell surface staining of mouse TRAIL by FACS in combination with PAb to human IgG.
 
Reconstitution:Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that TRAIL-R2 inhibits mouse soluble TRAIL-induced apoptosis assessed in human B cells (BJAB) in a concentration range of 1-10µg/ml.
 
522-067
Method: 60ng/ml rmsSuperKillerTRAIL™ (Prod. No. ALX-201-130) were pre-incubated for 30 minutes at room temperature with the indicated concentration of recombinant mTRAIL-R2:Fc. (Prod. No. ALX-522-067). Human BJAB target cells (50,000 cells in 100µl RPMI medium containing 10% fetal calf serum) were then added and incubated O/N at 37°C in a 96 well plate. Cell viability was determined using a PMS/MTS-based cell proliferation assay test. % of apoptosis inhibition was calculated based on the OD measured at 490nm (OD measured with rmsSuperKillerTRAIL™-treated dead cells in the absence of mTRAIL-R2:Fc: 0% inhibition. OD measured in the presence of mTRAIL-R2:Fc leading to complete inhibition of apoptosis: 100% inhibition).
Please mouse over
522-067

Product Literature References

Pigment epithelium-derived factor (PEDF) promotes tumor cell death by inducing macrophage membrane tumor necrosis factor-related apoptosis-inducing ligand (TRAIL): T.C. Ho, et al.; J. Biol. Chem. 286, 35943 (2011), Application(s): In vivo inhibition of TRAIL-mediated apoptosis, Abstract; Full Text

General Literature References

Identification of a new murine tumor necrosis factor receptor locus that contains two novel murine receptors for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL): P. Schneider, et al.; J. Biol. Chem. 278, 5444 (2003), Abstract;
Molecular cloning and functional analysis of the mouse homologue of the KILLER/DR5 tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor: G.S. Wu, et al.; Cancer Res. 59, 2770 (1999), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
TRAIL receptor
By biological activity:
TRAIL receptor Recombinant protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Product Flyers
TRAIL & TRAIL Receptors
TRAIL & TRAIL Receptors
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,