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CCR8 (human) polyclonal antibody

 
ALX-210-764-C100 100 µg 287.00 USD
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Product Specification

Alternative Name:C-C chemokine receptor 8, CD198
 
Host:Goat
 
Immunogen:Synthetic peptide corresponding to aa 184-193 (Y184SFYNQQTLK193) of the 2nd extracellular domain of human CCR8 (C-C Chemokine Receptor 8).
 
UniProt ID:P51685
 
Species reactivity:Human
 
Applications:ICC, IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (acetone fixed cells (1:800))
Immunohistochemistry (paraffin sections (1:300))
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Epitope-affinity purified IgG.
 
Quality Control:Immunocytochemistry on human K562 cells.
 
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Protocol:Immunohistochemistry on paraffin sections:
- Fix paraffin sections in 10% formalin.
- De-wax sections and block endogenous peroxide by incubating sections with 3% H2O2 for 15 minutes at room temperature (RT).
-Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block the non-specific binding sites.
- Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 minutes at RT.
- Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 minutes.
- Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 minutes.
- Wash slides and incubate with DAB for 5-7 minutes at RT.
- Wash slides with PBS and counter stain with hematoxylin.
 

Product Literature References

Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL: N.E. Annels, et al.; Blood 103, 2806 (2004), Abstract;
Phenotype, localization, and mechanism of suppression of CD4(+)CD25(+) human thymocytes: F. Annunziato, et al.; J. Exp. Med. 196, 379 (2002), Abstract; Full Text
The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium: N.S. Haque, et al.; Blood 97, 39 (2001), Abstract; Full Text
CC Chemokine I-309 is the Principal Monocyte Chemoattractant Induced by Apolipoprotein(a) in Human Vascular Endothelial Cells: N.S. Haque, et al.; Circulation 102, 786 (2000), Abstract; Full Text
The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains: R. Horuk, et al.; J. Biol. Chem. 273, 386 (1998), Abstract; Full Text
Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor: M. Samson, et al.; Eur. J. Immunol. 26, 3021 (1996), Abstract;

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