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HIGHDEF® IHC enhancer (AP)

ADI-950-180-0250 250 ml Inquire for pricing
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The HIGHDEF® IHC enhancer (AP) is a signal enhancer that can be used in conjunction with alkaline phosphatase based immunostaining systems.

Product Specification

Formulation:Liquid. TRIS-based alkaline buffer with <0.1% sodium azide.
Appearance:Clear solution.
Long Term Storage:Ambient
Scientific Background:Alkaline Phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization and DNA sequencing. Detection of this enzyme requires the generation of an insoluble colored reaction end product. Stronger the signal, better the staining. We introduces a new stable solution to increase the signal generated by alkaline phosphatase several fold. A single treatment of tissue sections with enhancer before the treatment with substrate/chromogen enables the visualization of difficult to localize antigens. HIGHDEF® IHC enhancer (AP) works well with Fast Red but can be used with BCIP/NBT and BCIP/INT also. Specimens stained with the above substrates/chromogens can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium like HIGHDEF® IHC mount (Prod. no. ADI-950-261).
Technical Info/Product Notes:Interpretation of the results will be the sole responsibility of the user.
  1. Once tissue sections have been incubated with Streptavidin-Alkaline Phosphatase, wash thoroughly with wash buffer.
  2. Blot the slide(s) to remove excess wash buffer and add enough drops of HIGHDEF IHC enhancer (AP) to cover the tissue sections. Alternately, pour HIGHDEF IHC enhancer (AP) in a coplin jar and soak the slide(s).
  3. Incubate for 1 minute at room temperature.
  4. Drain excess HIGHDEF IHC enhancer (AP) and add substrate/chromogen on the tissue sections without any wash between the two steps.

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