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5-Methylcytosine monoclonal antibody (10G4)

ADI-905-903-0050 50 µg 219.00 USD
ADI-905-903-0200 200 µg 616.00 USD
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Product Specification

Alternative Name:5-mC
Species reactivity:Species independent
Specificity:Recognizes 5-methylcytosine in single stranded DNA and 5-methylcytidine from any source including human, rat, mouse, plants, etc. and 5-Methylcytidine.
Applications:ELISA, IP
Recommended Dilutions/Conditions:ELISA (1:1,000-1:4000)
Immunoblotting (1:500-1:5,000)
Immunoprecipitation (2-4 µg).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Purified.
Formulation:Liquid. In PBS, pH 7.4, containing 0.05% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The ability to detect and quantify DNA methylation (e.g. 5-methylcytosine) efficiently and accurately has become essential for the study of cancer, gene expression, genetic diseases, as well as many other important aspects of epigenetics. 5-Methylcytosine, mAb (10G4) can differentiation between methylated and non-methylated cytosines in DNA. Specificity of this clone is to 5-methylcytosines in single-stranded DNA with no detectable crossreactivity to non-methylated cytosines. The antibody has been shown to be a valuable tool in the characterization of DNA methylation and has been successfully used for immunopreciptiation-based assays such as Methylated DNA Immunoprecipitation (MeIP).
ADI-905-903 IP
Immunoprecipitation analysis: Methylated DNA is efficiently enriched using the 5-Methylcytosine mAb (10G4). DNA was immunoprecipitated using the 5-Methylcytosine, mAb (10G4) from a mixed methylated/non-methylated DNA population. This population is comprised of a mixture of non-methylated plasmid DNA and a methylated version of the same plasmid containing a point mutation that introduces an NcoI restriction site. After immunoprecipitation of the mixture, the region of DNA containing the restriction site was amplified by PCR, digested with NcoI, and visualized in a 2.0% (w/v) agarose/TAE/EtBr gel. Non-methylated DNA remains un-cut, whereas the methylated DNA is cut by NcoI. The image demonstrates specific enrichment of methylated versus non-methylated DNA by the 5-Methylcytosine mAb (10G4).
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ADI-905-903 IP

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