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Dkk-1 (rat), ELISA kit

First-to-market rat Dkk-1 ELISA commercially available for wnt signaling pathway research.
 
ADI-900-171 96 wells 494.00 USD
Do you need bulk/larger quantities?
 
  • Easy-to-use - ready-to-use liquid color-coded reagents reduce errors
  • Sensitive - detect as little as 26.1 pg/ml
  • Accurate - fully quantitative results surpass semi-quantative Western blot analysis
The Dkk-1 (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. This kit has low reactivity to Dickkopf-1 family members and offers fully-quantitative and reproducible results for a low cost-per-test.

Product Specification

Alternative Name:Dickkopf-1
 
Sensitivity:26.1 pg/ml (range 39.1 - 1250 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of rat Dkk-1 in culture supernatants, plasma, and serum.
 
Species reactivity:Rat
 
Crossreactivity:Rat Dkk-1 (100%) and <0.03%: mouse Dkk-2, Dkk-4, Sgy-1
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 36, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:Dkk-1 (Dickkopf-1) is a secreted inhibitor of the canonical Wnt signaling pathway, which mediates a variety of cellular processes including the growth and development of osteoblasts. Dkk-1 disrupts the canonical pathway by binding to the LRP5/6 co-receptors, preventing interaction with Wnt, and facilitating the degradation of beta-catenin. Dkk-1 is overexpressed in multiple myeloma, increasing bone resorption and the appearance of osteolytic lesions characteristic of multiple myeloma.
 

Product Literature References

Traumatic brain injury result in accelerated fracture healing: a rat model: Y. Chen, et al.; Journal of Clinical and Experimental Orthopaedics 1, Article number 6 (2015), Application(s): In vivo chemotaxis assay, Full Text

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For Research Use Only. Not for use in diagnostic procedures.
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