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Oxytocin ELISA kit

The most sensitive, specific and flexible alternative to more costly and time-consuming methods for oxytocin detection.
 
ADI-900-153A-0001 96 wells 425.00 USD
 
Replaces Prod. #: ADI-900-153

Alternative size available: ADI-901-153A (5x96 wells)
  • Specific - does not detect vasopressin, providing confidence in assay results
  • Sensitive - detect 15pg/ml oxytocin
  • Cost effective – faster and less costly than LC/MS
  • Easy-to-use - liquid color-coded reagents and pre-coated plates reduce errors
  • Trusted - highly published in peer reviewed literature
The Oxytocin EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour. Absorbance is read at 405 nm. Very low reactivity with vasopressin provides confidence in assay results. Sensitive quantification of 15pg/mL oxytocin. Ready-to-use liquid color-coded reagents reduce errors in the lab.
ADI-900-153A std curve
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ADI-900-153A std curve

Product Specification

Sensitivity:15pg/ml (range 15.6 - 1,000 pg/ml)
 
Assay Time:Overnight + 1 hour
 
Application:For the quantitative determination of Oxytocin in culture supernatants, milk, plasma, and serum from any species. Cited sample types include cerebral spinal fluid, saliva, tissue and urine.
 
Crossreactivity:Mesotocin (7%), Oxytocin (100%), Arg8-Vasotocin (7.5%), and <0.02% for other related molecules.
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Miscellaneous/General:Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior, and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.
 

Product Literature References

Effects of early life social stress on endocrinology, maternal behavior, and lactation in rats: L.M. Carini, et al.; Horm. Behav. 64, 634 (2013), Application(s): EIA using rat plasma, Abstract;
Neonatal CSF oxytocin levels are associated with parent report of infant soothability and sociability: C.L. Clark, et al.; Psychoneuroendocrinology 38, 1208 (2013), Application(s): ELISA using human cerebral spinal fluid., Abstract;
Oxytocin increases the influence of public service advertisements: P.Y. Lin, et al.; PLoS One 8, e56934 (2013), Abstract;
Peripheral vasopressin but not oxytocin relates to severity of acute psychosis in women with acutely-ill untreated first-episode psychosis: L.H. Rubin, et al.; Schizophr. Res. 146, 138 (2013), Application(s): ELISA using human serum., Abstract;
Oxytocin administration attenuates atherosclerosis and inflammation in Watanabe Heritable Hyperlipidemic rabbits: A. Szeto, et al.; Psychopharmacol. (2012), Abstract;
Oxytocin and vasopressin are dysregulated in Williams Syndrome, a genetic disorder affecting social behavior: L. Dai, et al.; PLoS One 7, e38513 (2012), Application(s): ELISA using human serum, Abstract; Full Text
Peripheral oxytocin in female baboons relates to estrous state and maintenance of sexual consortships: L.R. Moscovice, et al.; Horm. Behav. 62, 592 (2012), Application(s): ELISA using baboon urine, Abstract;
Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress, and energetic metabolism biomarkers in urine: K.G. Salvante, et al.; Am. J. Hum. Biol. 24, 81 (2012), Application(s): EIA using human urine, Abstract; Full Text
Estradiol effects on behavior and serum oxytocin are modified by social status and polymorphisms in the serotonin transporter gene in female rhesus monkeys: V. Michopoulos, et al.; Horm. Behav. 59, 528 (2011), Application(s): ELISA using rhesus monkey serum, Abstract;
Evaluation of Enzyme Immunoassay and Radioimmunoassay Methods for the Measurement of Plasma Oxytocin: A. Szeto, et al.; Psychosomatic Medicine 73, 393 (2011), Abstract;
Genistein alters the release of oxytocin, prostaglandins, cortisol and LH during insemination in gilts: M. Norrby, et al.; Reprod. Domest. Anim. 46, 316 (2011), Application(s): ELISA using pig plasma, Abstract;
Reproductive experience affects parental retrieval behaviour associated with increased plasma oxytocin levels in wild-type and CD38-knockout mice: O. Lopatina, et al.; J. Neuroendocrinol. 23, 1125 (2011), Abstract;
Calcium, parathyroid hormone, oxytocin and pH profiles in the whelping bitch: F.K. Hollinshead, et al.; Theriogenology 73, 1276 (2010), Application(s): ELISA using dog serum, Abstract;
Mothers’ and Children’s Concentrations of Oxytocin Following Close, Physical Interactions with Biological and Non-biological Children: J. Bick & M. Dozier; Dev. Psychobiol. 52, 100 (2010), Application(s): Concentration in urine, Abstract; Full Text
Prolactin promotes oxytocin and vasopressin release by activating neuronal nitric oxide synthase in the supraoptic and paraventricular nuclei: C. Vega, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 299, R1701 (2010), Application(s): ELISA using rat plasma, Abstract; Full Text
The cross-generation transmission of oxytocin in humans: R. Feldman, I. Gordon, & O. Zagoory-Sharon; Hormones and Behavior 58, 669 (2010), Application(s): EIA using human saliva (ref. 4)., Abstract;
Variation in oxytocin is related to variation in affiliative behavior in monogamous, pairbonded tamarins: C.T. Snowdon, et al.; Horm. Behav. 58, 614 (2010), Application(s): ELISA using tamarin urine, Abstract;
Effects of naltrexone on cortisol levels in heavy drinkers: L.A. Ray, et al.; Pharmacol. Biochem. Behav. 91, 489 (2009), Application(s): EIA using human saliva, Abstract;
Influence of a 'Warm Touch' Support Enhancement Intervention Among Married Couples on Ambulatory Blood Pressure, Oxytocin, Alpha Amylase,and Cortisol: J. Holt-Lunstad, et al. ; Psychosom. Med. 70, 976 (2008), Application(s): EIA using human plasma and saliva (ref. 2), Abstract;
Monetary sacrifice among strangers is mediated by endogenous oxytocin release after physical contact: P. Zak, et al. ; Evol. Hum. Behav. 29, 375 (2008), Application(s): EIA using human plasma and serum.,
PVN electrical stimulation prolongs withdrawal latencies and releases oxytocin in cerebrospinal fluid, plasma, and spinal cord tissue inintact and neuropathic rats: M. Condes-Lara, et al. ; Pain 140, 265 (2008), Application(s): EIA using rat cerbrospinal fluid, plasma and tissue (ref. 1), Abstract;
Oxytocin in the circadian timing of birth: J. Roizen, et al.; PLoS One 2, e922 (2007), Application(s): EIA using mouse plasma, Abstract; Full Text

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