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Arg8-Vasopressin ELISA kit

Highly sensitive ELISA kit for the detection of Arg8-Vasopressin in serum, plasma and tissue culture media.
ADI-901-017A 5x96 wells 2,062.00 USD
Do you need bulk/larger quantities?
Replaces Prod. #: ADI-901-017

Alternative size available: ADI-900-017A (1x96 wells)
  • High sensitivity, detecting as little as 2.84 pg/ml
  • Highly specific with negligible reactivity with oxytocin
  • Simple and easy-to-use liquid color-coded reagents reduces error
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The Arg8-Vasopressin EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour. Absorbance is read at 450 nm.
Arg8-Vasopressin ELISA kit Standard curve
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Arg8-Vasopressin ELISA kit Standard curve

Product Details

Alternative Name:AVP
Sensitivity:2.84 pg/ml (range 4.10 - 1,000 pg/ml)
Assay Time:Overnight + 1 hour
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of Arg8-Vasopressin in serum, plasma, and tissue culture media.
Wavelength:450 nm
Species reactivity:Species independent
Use/Stability:Store all components at +4°C, except standard at -20°C.
Shipping:Blue Ice
Contents:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, SA-HRP, TMB Substrate, Stop solution
Scientific Background:Arginine vasopressin (AVP) is a nonapeptide hormone produced in magnocellular neurons of the hypothalamus, which functions as an antidiuretic in the kidney and systemically as a regulator of vasoconstriction, blood volume, and blood pressure. AVP acts by binding to a set of specific seven transmembrane domain-containing G-protein coupled receptors (GPCR) classified into three groups: V1a (V1), V1b (V3), and V2. V1b receptors stimulate adrenocorticotropic hormone (ACTH) release in the anterior pituitary, but may also participate in corticotropin-releasing hormone (CRH) release by the hypothalamus. The V1b receptor signals through coupling of Gq/11 alpha subunits, activating phospholipase C and release of intracellular Ca2+ via phosphoinositide second messengers. V1b receptor overexpression is a common marker for ACTH-secreting corticotropic adenomas. V2 receptors in the collecting ducts of the kidneys stimulate adenylyl cyclase activity, regulating the reabsorption of water via aquaporin channels.
Technical Info/Product Notes:Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types
UniProt ID:P01185 (human)
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Contrasting central and systemic effects of arginine-vasopressin on urinary marking behavior as a social signal in male mice: Y. Higuchi, et al.; Horm. Behav. 141, 105128 (2022), Abstract;
Evaluating the neuropeptide-social cognition link in ageing: the mediating role of basic cognitive skills: R. Polk, et al.; Philos. Trans. R. Soc. Lond. B Biol. Sci. 29, 377 (2022), Abstract;
Persistent autism-relevant behavioral phenotype and social neuropeptide alterations in female mice offspring induced by maternal transfer of PBDE congeners in the commercial mixture DE-72: E.V. Kozlova, et al.; Arch. Toxicol. 96, 335 (2022), Abstract;
Renal function during sevoflurane or total intravenous propofol anaesthesia: a single-centre parallel randomised controlled study: S. Franzen, et al.; Br. J. Anaesth. 1289, 838 (2022), Abstract;
Transcription factor Creb3l1 maintains proteostasis in neuroendocrine cells: M. Greenwood, et al.; Mol. Metab. 63, 101542 (2022), Abstract;
Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin: J. Bigalke, et al.; Front. Physiol. 12, 641331 (2021), Abstract; Full Text
Autism-associated biomarkers: test–retest reliability and relationship to quantitative social trait variation in rhesus monkeys: O. Oztan, et al.; Mol. Autism 12, 13229 (2021), Abstract;
Circadian Clock Component BMAL1 in the Paraventricular Nucleus Regulates Glucose Metabolism: M. Nakata, et al.; Nutrients 13, 4487 (2021), Abstract;
Differential roles of VPS and RAAS in water homeostasis and a risk for kidney dysfunction in rats undergoing rapid fasting/dehydration with regular exercise: K. Hasegawa, et al.; Physiol. Rep. 9, e14670 (2021), Application(s): ELISA using rat plasma, Abstract; Full Text
Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure: Y. Funato, et al.; Nat. Commun. 12, 3683 (2021), Application(s): ELISA using mouse plasma, Abstract; Full Text
Loss of Soluble (Pro)renin Receptor Attenuates Angiotensin-II Induced Hypertension and Renal Injury: N. Ramkumar, et al.; Circ. Res. 129, 50 (2021), Application(s): Mouse urine, Abstract;
Oestrogen inhibits salt-dependent hypertension by suppressing GABAergic excitation in magnocellular AVP neurons: X. Jin, et al.; Cardiovasc. Res. 117, 2263 (2021), Application(s): ELISA using rat plasma, Abstract;
Purinergic signaling is enhanced in the absence of UT-A1 and UT-A3: N.J. Himmel, et al.; Physiol. Rep. 6, e14636 (2021), Application(s): ELISA using mouse serum, Abstract; Full Text
Renal inflammation induces salt sensitivity in male db/db Mice through dysregulation of ENaC: L.C. Veiras, et al.; J. Am. Soc. Nephrol. 32, 1131 (2021), Application(s): ELISA using mouse plasma, Abstract;
Sex-dependent Effects of Nephron Ift88 Disruption on Blood Pressure, Renal Function and Cystogenesis: C. Hu, et al.; J. Am. Soc. Nephrol. 32, 2210 (2021), Abstract;
The Mechanism of the Solute-Free Water Reabsorption Increase in the Rat Kidney by Oxytocin Saluresis: Y.V. Natochin, et al.; Dokl Biochem Biophys 497, 190 (2021), Abstract;
Use of Cocultures to Measure the Blood–Brain Barrier Permeability of Oxytocin: Y. Yamamoto, et al.; Methods Mol. Biol. 2384, 247 (2021), Abstract;
Genetic deletion of connexin 37 causes polyuria and polydipsia: J. Xue, et al.; PLoS One 15, e0244251 (2020), Abstract; Full Text
Neonatal CSF vasopressin concentration predicts later medical record diagnoses of autism spectrum disorder: O. Oztan, et al.; PNAS 117, 10609 (2020), Abstract; Full Text
A randomized controlled trial comparing methohexital and propofol for induction in patients receiving angiotensin axis blockade: A. Bonavia, et al.; Medicine (Baltimore) 98, e14374 (2019), Application(s): used on human blood serum samples- measure neurohormone concentrations of CORT, Abstract; Full Text
Chronic water insufficiency induced kidney damage and energy dysregulation despite reduced food intake, which improved gut microbiota in female rats: J.W. Daily, et al.; J. Physiol. Sci. 69, 599 (2019), Application(s): Rat Serum, Abstract;
Effects of Environmental Enrichment in Maternally Separated Rats: Age and Sex-Specific Outcomes: R. Doreste, et al.; Front. Behav. Neurosci. 13, 198 (2019), Abstract;
Sickle cell disease up-regulates vasopressin, aquaporin 2, urea transporter A1, Na-K-Cl cotransporter 2, and epithelial Na channels in the mouse kidney medulla despite compromising urinary concentration ability: H. Wang, et al.; Physiol. Rep. 7, e14066 (2019), Abstract; Full Text
BDNF levels are associated with autistic traits in the general population: N. Brondino, et al.; Psychoneuroendocrinology 89, 131 (2018), Application(s): Blood Samples, Abstract;
The effects of aging on biosynthetic processes in the rat hypothalamic osmoregulatory neuroendocrine system: M.P. Greenwood, et al.; Neurobiol. Aging 65, 178 (2018), Application(s): ELISA using tissue extracts, Abstract; Full Text
Validation of salivary oxytocin and vasopressin as biomarkers in domestic dogs: E.L. MacLean, et al.; J. Neurosci. Methods 293, 67 (2018), Application(s): Vasopressin levels in dog saliva, Abstract;
Biomarker discovery for disease status and symptom severity in children with autism: O. Oztan, et al.; Psychoneuroendocrinology 89, 39 (2017), Abstract;
Characterization of Brain-Heart Interactions in a Rodent Model of Sepsis: B.B. Pinto, et al.; Mol. Neurobiol. 54, 3745 (2017), Application(s): Rat plasma vasopressin levels were measured, Abstract; Full Text
Sex differences in associations of arginine vasopressin and oxytocin with resting-state functional brain connectivity: L.H. Rubin, et al.; J. Neurosci. Res. 95, 576 (2017), Application(s): Human serum samples, Abstract; Full Text
Supplemental arginine vasopressin during the resuscitation of severe hemorrhagic shock preserves renal mitochondrial function: C.A. Sims, et al.; PLoS One 12, e0186339 (2017), Abstract; Full Text
Comparing oxytocin and cortisol regulation in a double-blind, placebo-controlled, hydrocortisone challenge pilot study in children with autism and typical development: B.A. Corbett, et al.; J. Neurodev. Disord. 8, 32 (2016), Application(s): AVP assay, human studies, Abstract; Full Text
Prenatal Testosterone Exposure Decreases Aldosterone Production but Maintains Normal Plasma Volume and Increases Blood Pressure in Adult Female Rats: A.S. More, et al.; Biol. Reprod. 95, 42 (2016), Application(s): Plasma AVP levels, Abstract; Full Text
Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathway: H.J. Sun, et al.; Neuropharmacology 103, 247 (2016), Application(s): Measurement of plasma AVP, Abstract;
Serotonin 1A agonism decreases affiliative behavior in pair-bonded titi monkeys: R.H. Larke, et al.; Horm. Behav. 86, 71 (2016), Application(s): Blood sampling and hormone analysis, titi monkey plasma, Abstract;
Thirst responses following high intensity intermittent exercise when access to ad libitum water intake was permitted, not permitted or delayed: S.A. Mears, et al.; Physiol. Behav. 157, 47 (2016), Application(s): Measured arginine vasopressin in plasma, Abstract;
Arginine Vasopressin Is a Blood-Based Biomarker of Social Functioning in Children with Autism: D.S. Carson, et al.; PLoS One 10, e132224 (2015), Abstract; Full Text
Clopidogrel attenuates lithium-induced alterations in renal water and sodium channels/transporters in mice: Y. Zhang, et al.; Purinergic Signal. 11, 507 (2015), Application(s): Levels of vasopressin in mouse urine, Abstract;
Control of Polyamine Biosynthesis by Antizyme Inhibitor 1 is Important for Transcriptional Regulation of Arginine Vasopressin in the Male Rat Hypothalamus: M.P. Greenwood, et al.; Endocrinology 156, 2905 (2015), Application(s): ELISA using rat plasma, Abstract; Full Text
Effects of hydration in rats and mice with polycystic kidney disease: K. Hopp, et al.; Am. J. Physiol. Renal Physiol. 308, F261 (2015), Application(s): Levels of vasopressin in mouse and rat urine, Abstract;
Electro-acupuncture improves the social interaction behavior of rats: H.F. Zhang, et al.; Physiol. Behav. 151, 485 (2015), Application(s): ELISA using rat brain tissue, Abstract;
Explicit and implicit caregiving interests in expectant fathers: Do endogenous and exogenous oxytocin and vasopressin matter?: C.C. Cohen-Bendahan, et al.; Infant Behav. Dev. 41, 26 (2015), Application(s): Levels of vasopressin in human urine, Abstract;
Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi2 proteins: C. Y. Carmichael, et al.; Acta Physiol. 216, 258 (2015), Application(s): Rat plasma, Abstract; Full Text
Intraocular pressure following 18 hours of systemic dehydration in ocular normotensive healthy subjects: F.K. Idu, et al.; Afr. Vision Eye Health 74, 10 (2015), Application(s): ELISA using human plasma, Full Text
NSAIDs alter phosphorylated forms of AQP2 in the inner medullary tip: H. Ren, et al.; PLoS One 10, e0141714 (2015), Application(s): Levels of vasopressin in rat serum, Abstract; Full Text
Regulation of the Na+‐K+‐2Cl− cotransporter by cGMP/cGMP‐dependent protein kinase I after furosemide administration: F. Limmer, et al.; FEBS J. 282, 3786 (2015), Application(s): Mouse urine, Abstract; Full Text
Rho GAP myosin IXa is a regulator of kidney tubule function: S. Thelen, et al.; Am. J. Physiol. Renal Physiol. 309, F501 (2015), Application(s): To determine antidiuretic hormone (ADH) levels in urine and blood plasma of WT and Myo9a−/− mice, Abstract;
Breaking bonds in male prairie vole: long-term effects on emotional and social behavior, physiology, and neurochemistry: P. Sun, et al.; Brain Behav. Immun. 15, 265 (2014), Application(s): EIA using prairie vole plasma samples, Abstract;
Function of cGMP-dependent protein kinase II in volume load-induced diuresis: A. Schramm, et al.; Pflugers Arch. 466, 2009 (2014), Application(s): Mouse urine, Abstract;
P2X7 receptors in neurohypophysial terminals: evidence for their role in arginine-vasopressin secretion: A.E. Cuadra, et al.; J. Cell. Physiol. 229, 333 (2014), Abstract; Full Text
Transcription factor CREB3L1 regulates vasopressin gene expression in the rat hypothalamus: M. Greenwood, et al.; J. Neurosci. 34, 3810 (2014), Application(s): EIA using rat plasma and pituitary tissue samples, Abstract; Full Text
Excess maternal salt intake produces sex-specific hypertension in offspring: putative roles for kidney and gastrointestinal sodium handling: C. Gray, et al.; PLoS One 8, e72682 (2013), Application(s): EIA using rat plasma, Abstract; Full Text
Peripheral vasopressin but not oxytocin relates to severity of acute psychosis in women with acutely-ill untreated first-episode psychosis: L.H. Rubin, et al.; Schizophr. Res. 146, 138 (2013), Application(s): ELISA using human serum., Abstract;
Salivary vasopressin increases following intranasal oxytocin administration: O. Weisman, et al.; Peptides 40, 99 (2013), Application(s): ELISA using human saliva, Abstract;
Defective renal water handling in transgenic mice over-expressing human CD39/NTPDase1: Y. Zhang, et al.; Am. J. Physiol. Renal Physiol. 303, F420 (2012), Application(s): ELISA using mouse urine, Abstract;
Oxytocin and vasopressin are dysregulated in Williams Syndrome, a genetic disorder affecting social behavior: L. Dai, et al.; PLoS One 7, e38513 (2012), Application(s): ELISA using human serum, Abstract;
Physiological function of the angiotensin AT1a receptor in bone remodeling: K. Kaneko, et al.; J. Bone Miner. Res. 26, 2959 (2011), Application(s): ELISA using mouse serum, Abstract;
Prolactin promotes oxytocin and vasopressin release by activating neuronal nitric oxide synthase in the supraoptic and paraventricular nuclei: C. Vega, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 299, R1701 (2010), Application(s): ELISA using rat plasma, Abstract; Full Text
Endogenous ATP potentiates only vasopressin secretion from neurohypophysial terminals: J. Lemos, et al. ; J. Cell. Physiol. 217, 155 (2008), Application(s): EIA using rat culture supernatants, Abstract;
Modulation of aquaporin-2/vasopressin2 receptor kidney expression andtubular injury after endotoxin (lipopolysaccharide) challenge: F. Chagnon, et al.; Crit. Care Med. 36, 3054 (2008), Application(s): EIA using plasma, Abstract; Full Text
Tachykinin neurokinin 3 receptor signaling in cholecystokinin-elicited release of oxytocin and vasopressin: G.E. Haley & F.W. Flynn; Am. J. Physiol. Regul. Integr. Comp. Physiol. 294, R1760 (2008), Application(s): EIA using plasma, Abstract;
Hormonal correlates of human paternal interactions: A hospital-based investigation in urban Jamaica: P.B. Gray, et al.; Horm. Behav. 52, 499 (2007), Application(s): EIA using human urine, Abstract;
Loss of Vav2 proto-oncogene causes tachycardia and cardiovascular disease in mice: X. Bustelo, et al. ; Mol. Biol. Cell 18, 943 (2007), Application(s): EIA using mouse plasma, Abstract;
Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge: G.E. Haley, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R931 (2007), Application(s): EIA using rat plasma, Abstract; Full Text
Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension: J.D. Klein, et al. ; Ren. Physiol. 291, F952 (2006), Application(s): EIA using rat plasma, Abstract;

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