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Excellent sensitivity
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Validated for use with human samples
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Stable color-coded reagents reduce errors
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Save with bulk kit package!
A highly sensitive competitive immunoassay for the measurement of Testosterone in human serum and plasma samples. Results are read on a colorimetric plate reader at 405 nm.
Product Details
| Sensitivity: | 2.6 pg/ml (range 3.9 - 1000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human testosterone in plasma, serum, and urine samples. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Human
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Contents: | GxM Microtiter plate, Assay buffer, Standard, Antibody, Conjugate, Wash buffer concentrate, pNpp Substrate, Stop solution |
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| Scientific Background: | Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is the main androgen secreted by the Leydig cells of the testes and effects both primary and secondary sexual development such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Structural and functional changes in rat uterus induced by neonatal androgenization: R.C. Genaro, et al.; J. Mol. Histol.
53, 903 (2022),
Abstract;
Increased testosterone and proinflammatory cytokines in patients with polycystic ovary syndrome correlate with elevated GnRH receptor autoantibody activity assessed by a fluorescence resonance energy transfer-based bioassay: H. Li, et al.; Endocrine
74, 163 (2021),
Abstract;
Social instability influences rank-specific patterns of oxidative stress in a cichlid fish: S.E. Border, et al.; J. Exp. Biol.
224, 237172 (2021),
Abstract;
Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf.
225, 112771 (2021),
Abstract;
Testosterone production and social environment vary with breeding stage in a competitive female songbird: E.M. George, et al.; Horm. Behav.
103, 28 (2018),
Abstract;
Dysregulation of Notch and ERα signaling in AhR-/- male mice: B. Huang, et al.; PNAS
18, 113 (2016),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
The early evolving sex hormone environment is associated with significant outcome and inflammatory response differences after injury: S.J. Zolin, et al.; J. Trauma Acute Care Surg.
78, 451 (2015),
Application(s): ELISA using human plasma,
Abstract;
Full Text
Of mice and men--warning: intact versus castrated adult male mice as xenograft hosts are equivalent to hypogonadal versus abiraterone treated aging human males, respectively: J.P. Michiel Sedelaar, et al.; Prostate
73, 1316 (2013),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
General Literature References
Daily rhythmic and non-rhythmic variations of follitropin, lutropin, testosterone, and sex-hormone-binding globulin in men: J. Valero-Politi & X. Fuentes-Arderiu; Eur. J. Clin. Chem. Clin. Biochem.
34, 455 (1996),
Abstract;
Reproductive Endocrinology: R.J. Santen; W.B. Saunders 739-794 (1992),
Williams Textbook of Endocrinology 8th Ed.: J.E. Griffen & J.D. Wilson; W.B. Saunders 799-852 (1992),
Infertility, Contraception & Reproductive Endocrinology: F.Z. Stanczyk; Blackwell 53-76 (1991),
The Physiology of Reproduction: P.F. Hall; Raven Press 975-998 (1988),
M.L. Dufua, et al.; J. Steroid. Biochem. 20, 161 (1984),
The hormonal control of sexual development: J.D. Wilson, et al.; Science
211, 1278 (1981),
Abstract;
Steroid 5alpha-reductase deficiency in man: an inherited form of male pseudohermaphroditism: J. Imperato-McGinley, et al.; Science
186, 1213 (1974),
Abstract;
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