Product Details
| Alternative Name: | CRYAB, HSPB5 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of human αB-Crystallin. The sequence is completely conserved in rat, rabbit, and bovine. |
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| UniProt ID: | P02511 |
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| GenBank ID: | M28638 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Porcine
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| Applications: | IF, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~20kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of αB-Crystallin: Lane 1: MW marker, Lane 2:α-Crystallin (bovine), (native) (Prod No. ADI-SPP-225), Lane 3: αA-Crystallin (bovine), (native) (Prod No. ADI-SPP-226), Lane 4:αB-Crystallin (bovine), (native) (Prod No. ADI-SPP-227), Lane 5: β-Crystallin (bovine), (native) (Prod No. ADI-SPP-235)
Immunohistochemistry analysis of human brain, cortex tissue stained with αB-Crystallin, pAb at 10µg/ml.
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Product Literature References
Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion: H. Liu, et al.; Invest. Ophthalmol. Vis. Sci.
63, 3 (2022),
Abstract;
Overexpression of human BAG3P209L in mice causes restrictive cardiomyopathy: K. Kimura, et al.; Nat. Commun.
12, 3575 (2021),
Abstract;
Full Text
Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy: M. Schuldt, et al.; Front. Cardiovasc. Med.
8, 612215 (2021),
Abstract;
Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network: W.I.M. Vonk, et al.; Mol. Cell
78, 328 (2020),
Abstract;
HspB5 protects mouse neural stem/progenitor cells from paraquat toxicity: N. Mekala, et al.; Am. J. Stem Cells
9, 68 (2020),
Abstract;
p62/Sequestosome 1 levels increase and phosphorylation is altered in Cx50D47A lenses, but deletion of p62/sequestosome 1 does not improve transparency: O. Jara, et al.; Mol. Vis.
26, 204 (2020),
Abstract;
Full Text
Histone deacetylase inhibitor SAHA treatment prevents the development of heart failure after myocardial infarction via an induction of heat-shock proteins in rats: S. Nagata, et al.; Biol. Pharm. Bull.
42, 453 (2019),
Abstract;
Transcription Factor EB Activation Rescues Advanced αB-Crystallin Mutation-Induced Cardiomyopathy by Normalizing Desmin Localization: X. Ma, et al.; J. Am. Heart Assoc.
8, e010866 (2019),
Abstract;
A specific phosphorylation regulates the protective role of αA-crystallin in diabetes: A. Ruebsam, et al.; JCI Insight
3, e97919 (2018),
Abstract;
Intra-vitreal αB crystallin fused to elastin-like polypeptide provides neuroprotection in a mouse model of age-related macular degeneration: P.G. Sreekumar, et al.; J. Control Release
283, 94 (2018),
Abstract;
Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro: P. Murphy, et al.; Development
145, dev155838 (2018),
Abstract;
Full Text
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients: M. Wattin, et al.; Int. J. Mol. Sci.
19, 178 (2018),
Application(s): Western Blot,
Abstract;
Full Text
Docosahexaenoic acid-mediated protein aggregates may reduce proteasome activity and delay myotube degradation during muscle atrophy in vitro: S.K. Shin, et al.; Exp. Mol. Med.
49, e287 (2017),
Abstract;
Full Text
HSPB7 is indispensable for heart development by modulating actin filament assembly: T. Wu, et al.; PNAS
114, 11956 (2017),
Abstract;
Full Text
Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination: H.F. Kuipers, et al.; PNAS
114, E1745 (2017),
Abstract;
Full Text
Upregulation and phosphorylation of HspB1/Hsp25 and HspB5/αB-crystallin after transient middle cerebral artery occlusion in rats: B. Bartelt-Kirbach, et al.; Cell Stress Chaperones
22, 653 (2017),
Abstract;
Full Text
Disruption of desmin-mitochondrial architecture in patients with regurgitant mitral valves and preserved ventricular function: M.I. Ahmed, et al.; J. Thorac. Cardiovasc. Surg.
152, 1059 (2016),
Abstract;
Full Text
Induction of expression and phosphorylation of heat shock protein B5 (CRYAB) in rat myometrium during pregnancy and labour: J.G. Nicoletti, et al.; Reproduction
152, 69 (2016),
Application(s): Western-blot and immunofluorescence using rat myometral tissue and hTERT-HM cells,
Abstract;
Regulation of c-Maf and αA-Crystallin in Ocular Lens by Fibroblast Growth Factor Signaling: Q. Xie, et al.; J. Biol. Chem.
291, 3947 (2016),
Abstract;
Full Text
Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes: E. Tian, et al.; Cell Rep.
16, 781 (2016),
Abstract;
Epileptogenic but MRI-normal perituberal tissue in Tuberous Sclerosis Complex contains tuber-specific abnormalities: A. A. Sosunov, et al.; Acta Neuropathol. Commun.
3, 17 (2015),
Application(s): Immunohistochemistry,
Abstract;
Full Text
Inhibition of HDAC6 modifies tau inclusion body formation and impairs autophagic clearance: J. Leyk, et al.; J. Mol. Neurosci.
55, 1031 (2015),
Abstract;
Selective and Compartmentalised Myelin Expression of HspB5: S. Quraishe, et al.; Neuroscience
316, 130 (2015),
Application(s): Immunohistochemistry,
Abstract;
The regulation of connective tissue growth factor expression influences the viability of human trabecular meshwork cells: S. Kuespert, et al.; J. Cell. Mol. Med.
19, 1010 (2015),
Application(s): Western Blotting, Immunohistochemistry,
Abstract;
Full Text
HSP90 modulates actin dynamics: inhibition of HSP90 leads to decreased cell motility and impairs invasion: A. Taiyab, et al.; Biochim. Biophys. Acta
1813, 213 (2011),
Application(s): Western-blot using F111 and MDA-MB-231 cell lines,
Abstract;
Kinetics of the translocation and phosphorylation of αB-crystallin in mouse heart mitochondria during ex vivo ischemia: R. Whittaker, et al.; Am. J. Physiol. Heart Circ. Physiol.
296, H1633 (2009),
Application(s): Immunofluorescence using mouse heart tissue sections,
Abstract;
Full Text
Localization of phosphorylated αB-crystallin to heart mitochondria during ischemia-reperfusion: J.K. Kin, et al.; Am. J. Physiol. Heart Circ. Physiol.
294, H337 (2008),
Application(s): Western-blot using mouse heart lysates,
Abstract;
alpha-Crystallin distribution in retinal pigment epithelium and effect of gene knockouts on sensitivity to oxidative stress: D. Hinton, et al. ; Mol. Vis.
13, 566 (2007),
Application(s): WB, IF using human tissue culture & cell lysates,
Abstract;
Mimicking phosphorylation of alphaB-crystallin on serine-59 is necessary and sufficient to provide maximal protection of cardiac myocytes from apoptosis: C.C. Glembotski, et al. ; Circ. Res.
92, 203 (2003),
Application(s): WB using rat samples,
Abstract;
Expression and induction of the stress protein alpha-B-crystallin in vascular endothelial cells: D. Drenckhahn, et al. ; Histochem. Cell Biol.
117, 203 (2002),
Application(s): WB using human & porcine samples,
Abstract;
Effects of body temperature during exercise training on myocardial adaptations: J.W. Starnes, et al. ; Am. J. Physiol. Heart Circ. Physiol.
280, H2271 (2001),
Application(s): WB using rat samples,
Abstract;
Mutant Cu/Zn-superoxide dismutase proteins have altered solubility and interact with heat shock/stress proteins in models of amyotrophic lateral sclerosis: H.D. Durham, et al. ; J. Biol. Chem.
276, 12791 (2001),
Application(s): WB, IP using mouse samples,
Abstract;
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