Optimized buffer system for efficient amplification
Enhancers and stabilizers improve reaction sensitivity and specificity
AMPIGENE® qPCR Green Mixes use the latest developments in polymerase technology and buffer chemistry to deliver high performing dye-based real-time PCR with minimal optimization. AMPIGENE® qPCR Green Mixes use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE® qPCR Green Mixes come with either Lo-ROX or Hi-ROX concentration. For Lo-ROX, see Prod. No. ENZ-NUC103. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.)
Instrument compatibility for Rox dye.
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Product Details
Applications:
PCR
Application Notes:
For dye-based qPCR
Shipping:
Blue Ice
Long Term Storage:
-20°C
Contents:
2x AMPIGENE® qPCR Green Mix Hi-ROX (includes dNTPs and polymerases)
Regulatory Status:
RUO - Research Use Only
Product Literature References
Helicobacter pylori Eradication Can Reverse Rho GTPase Expression in Gastric Carcinogenesis: J.L. Ki, et al.; Gut Liver (2023), Abstract;
Effect of PAIP1 on the metastatic potential and prognostic significance in oral squamous cell carcinoma: N. Swarp, et al.; Int. J. Oral Sci. 14, 9 (2022), Abstract;
Gene expression of adipokines and inflammatory cytokines in peripheral blood mononuclear cells of obese dogs: S.Y. Hwang, et al.; Vet. Med. Sci. 8, 517 (2022), Abstract;
Influenza A pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines: J. Lee, et al.; Vet. Immunol. Immunopathol. 246, 110404 (2022), Abstract;
Response of soybean (Glycine max L.) seedlings to polystyrene nanoplastics: Physiological, biochemical, and molecular perspectives: Y. Surgun-Acar; Environ. Pollut. 314, 10262 (2022), Abstract;
Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization: H.W. Jang, et al.; PLoS One 16, e0256651 (2021), Abstract;
Cip2A modulates osteogenic differentiation via the ERK-Runx2 pathway in MG63 cells: H.E. Son, et al.; Biofactors 47, 658 (2021), Abstract;
Extracellular vesicles derived from DFO-preconditioned canine AT-MSCs reprogram macrophages into M2 phase: S.M. Park, et al.; PLoS One 16, e0254657 (2021), Abstract;
Feline adipose tissue-derived mesenchymal stem cells pretreated with IFN-γ enhance immunomodulatory effects through the PGE2 pathway: S.G. Park, et al.; J. Vet. Sci. 22, e16 (2021), Application(s): Feline adipose-tissue derived (fAT)-MSCs; quantification of Cytokine mRNA levels, Abstract; Full Text
Localized Photothermal Ablation Therapy of Obstructive Rectal Cancer Using a Nanofunctionalized Stent in a Mouse Model: H.T. Hu, et al.; ACS Biomater. Sci. Eng. 7, 5890 (2021), Abstract;
Measuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile: J.Y. Kim, et al.; Microbiologyopen 10, e1220 (2021), Abstract;
Senotherapeutic-like effect of Silybum marianum flower extract revealed on human skin cells: J. Woo, et al.; PLoS One 16, e0260545 (2021), Abstract;
Alpha-pinene promotes osteoblast differentiation and attenuates TNFα-induced inhibition of differentiation in MC3T3-E1 pre-osteoblasts: H.Y. Min, et al.; Clin. Exp. Pharmacol. Physiol. 47, 831 (2020), Abstract;
TNF-α and INF-γ primed canine stem cell-derived extracellular vesicles alleviate experimental murine colitis: J.H. An, et al.; Sci. Rep. 10, 2115 (2020), Abstract; Full Text
Prostaglandin E2 secreted from feline adipose tissue-derived mesenchymal stem cells alleviate DSS-induced colitis by increasing regulatory T cells in mice: J.H. An, et al.; BMC Vet. Res. 14, 354 (2018), Abstract; Full Text
TSG-6 secreted by human adipose tissue-derived mesenchymal stem cells ameliorates severe acute pancreatitis via ER stress downregulation in mice: Q. Li, et al.; Stem Cell Res. Ther. 9, 255 (2018), Abstract; Full Text
Anti-inflammatory Effects of Oct4/Sox2-overexpressing Human Adipose Tissue-derived Mesenchymal Stem Cells: Q. Li, et al.; In Vivo 31, 349 (2017), Abstract; Full Text
Autophagy plays a role in skeletal muscle mitochondrial biogenesis in an endurance exercise-trained condition: J.S. Ju, et al.; J. Physiol. Sci. 66, 417 (2016), Abstract;
Bmal1 induces osteoblast differentiation via regulation of BMP2 expression in MC3T3-E1 cells: H.Y. Min, et al.; Life Sci. 3205, 30464 (2016), Application(s): Quantifying mRNA expression, mouse MC3T3-E1 cells , Abstract;
Canine adipose tissue-derived mesenchymal stem cells ameliorate severe acute pancreatitis by regulating T cells in rats: H.W. Kim, et al.; J. Vet. Sci. 17, 539 (2016), Abstract;
Simultaneous inhibition of multiple oncogenic miRNAs by a multi-potent microRNA sponge: J. Jung, et al.; Oncotarget 6, 20370 (2015), Abstract; Full Text
