Membrane-free, Cell Migration or Invasion: perform studies without manipulating transmembrane inserts - observe live images of cell movement
Creative Assay Design: coat any ECM or BME on the plate to create a 2-D environment for cell migration or cell invasion assays.
Preserve Cell Morphology: eliminate need for cells to penetrate through a polycarbonate membrane; cells can move across a treated surface or within a user-applied ECM
Real-time Monitoring: monitor changes in cell structure in real-time throughout the experiment.
Versatile Detection: analyze cells treated with multiple fluorescent probes, labels or stains by using a microscope, digital imaging system or fluorescence microplate reader.
Reproducible Results: obtain greater reproducibility using the Cell Seeding Stoppers compared to wound healing or scratch assays.
Flexible: perform kinetic or endpoint assays without the use of special instrumentation.
The Cell migration assay, flex kit is a reproducible, sensitive, and flexible assay that can be used to monitor cell migration or cell invasion. The Cell migration assay, flex kit now gives researchers more control over designing a cell migration assay. Each Cell migration assy, flex kit contains the Cell Seeding Stoppers for creating a detection zone at the center of each well in a 96-well plate. Since the stoppers are not pre-inserted into the wells, researchers can coat the plate with an extracellular matrix (ECM) component to design their assay. Researchers may also apply 3-dimensional overlays in each well to watch how cells invade and respond to various compounds (chemokinesis).
The Cell migration assay, flex kit is designed to be used with any stain or labeling technique. Readout can be performed by microscopy or use of a microplate reader.
The Cell migration assay, flex kit has been designed for use with adherent cell cultures. This assay has been successfully used with 3T3-Swiss albino, HT-1080, HCEC, HUVEC, and MCF10A cell lines.
Fig 3. Cell migration data using cell tracking stain.
Fig 2. Schematic of Cell migration assay, flex kit.
Fig 1. Formats of Cell migration assays.
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Product Specification
Applications:
Fluorescence microscopy, Fluorescent detection
Application Notes:
2-D assay for investigating cell migration of adherent cell lines
Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with soluble E-cadherin from human placenta., ELISA, Flow Cytometry, IF, IHC (FS), IHC (PS), WB, FUNC | Print as PDF
Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of Wistar rat immunized with mouse teratocarcinoma cell line F9. Purified from serum-free cell culture supernatant., FUNC | Print as PDF
Obtained by fusing the P3-X63-Ag8 myeloma cell line with splenocytes of Donryu rat immunized with mouse liver E-cadherin fragment., IHC (FS), WB, FUNC | Print as PDF
Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7., ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), WB, FUNC | Print as PDF
