Ultra-sensitive measurement of GLP-1, detecting as little as 5.5 pg/ml
Negligible reactivity from similar GLP-1 forms
High throughput format with results in 3 hours for up to 40 samples in duplicate
Fully quantitative results that surpass semi-quantitative Western blot analysis
The AMP'D® GLP-1 ELISA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.
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Product Details
Alternative Name: Glucagon-like peptide-1
Sensitivity: 5.5 pg/ml (range 7.8-250 pg/ml)
Assay Time: 3 hours
Applications: ELISA, Colorimetric detection
Application Notes: For the quantitative determination of Glucagon-like peptide 1 (GLP-1) (7-36) amide in serum and plasma of human origin.
Wavelength: 495 nm
Species reactivity: Human
Crossreactivity: GLP-1 (7-36) amide (100%), GLP-1 (7-37) (≤2.1%) and ≤0.02%: GLP-1 (9-36) amide, GLP-1 (1-36) amide, GLP-2, Glucagon
Use/Stability: Store all components at +4°C, except standard at -20°C.
Shipping: Blue Ice
Contents: Microtiter Plate, Assay Buffer 13, Standard, Wash Buffer Concentrate, Antibody, Conjugate, and AMP'D® (Signal Amplification) Substrate, Amplifier, Substrate Diluent, Amplifier Diluent, and Stop Solution
Scientific Background: GLP-1 functions in the regulation of blood glucose through insulin, and plays a role in obesity and diabetes. It is the most potent promoter of insulin secretion and an important gastrointestinal peptide hormone, or incretin. In response to eating, increased levels of GLP-1 stimulate insulin secretion and inhibit glucagon release leading to lower blood glucose levels. These positive effects of GLP-1 on glucose regulation make it an important target for the management of type 2 diabetes.
UniProt ID: P01275[98-127]
GenBank ID: X03991.1
Gene/Protein Identifier: NP_002045.1 (RefSeq), NM_002054.4 (RefSeq), 2641 (Entrez GeneID), 138030 (OMIM)
Regulatory Status: RUO - Research Use Only
Compatibility: This product is compatible with the Absorbance 96 Plate Reader .
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