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DnaK (E. coli) monoclonal antibody (8E2/2)

ENZ-ABS680-0200 200 µg 687.00 USD
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Product Details

Alternative Name:Hsp70
Immunogen:E. coli DnaK.
UniProt ID:P0A6Y8 (strain K12)
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:E. coli
Recommended Dilutions/Conditions:Western Blot (1:10,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~70kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
Regulatory Status:RUO - Research Use Only
DnaK (E. coli) monoclonal antibody (8E2/2) WB
Western Blot analysis of ENZ-ABS680: Lane 1: MW Marker, Lane 2: DnaK (E. coli) recombinant protein (Prod. No. ADI-SPP-630), Lane 3: E. coli cell lysate.
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DnaK (E. coli) monoclonal antibody (8E2/2) WB

Product Literature References

Genetic Programming by Nitric Oxide-Sensing Gene Switch System in Tumor-Targeting Bacteria: Y. Qin, et al.; Biosensors 13, 266 (2023), Abstract;
Omptin Proteases of Enterobacterales Show Conserved Regulation by the PhoPQ Two-Component System but Exhibit Divergent Protection from Antimicrobial Host Peptides and Complement: Y.H. Cho, et al.; Infect. Immun. 91, e00518 (2023), Abstract;
Transcriptional insight into the effect of benzalkonium chloride on resistance and virulence potential in Salmonella Typhimurium: S. Park & H. Yoon; Microbiol. Res. 266, 127240 (2023), Abstract;
OmpR and Prc contribute to switch the Salmonella morphogenetic program in response to phagosome cues: D.L. Escarpa, et al.; Mol. Microbiol. 118, 477 (2022), Abstract;
The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches: A. Hipólito, et al.; Nucleic Acids Res. 50, 8566 (2022), Abstract;

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