| Alternative Name: | Hsp70 |
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| Clone: | 8E2/2 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | E. coli DnaK. |
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| UniProt ID: | P0A6Y8 (strain K12) |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | E. coli
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:10,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~70kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes. |
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| Regulatory Status: | RUO - Research Use Only |
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