Purified from hybridoma tissue culture supernatant.
Species reactivity:
E. coli
Applications:
WB
Recommended Dilutions/Conditions:
Western Blot (1:10,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~70kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Scientific Background:
DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
Regulatory Status:
RUO - Research Use Only
Western Blot analysis of ENZ-ABS680: Lane 1: MW Marker, Lane 2: DnaK (E. coli) recombinant protein (Prod. No. ADI-SPP-630), Lane 3: E. coli cell lysate.
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Product Literature References
The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches: A. Hipólito, et al.; Nucleic Acids Res. 50, 8566 (2022), Abstract;
