MM 1-43 is a cationic dye, equivalent to membrane probe FM® 1-43. MM 1-43 is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The staining of cell-surface specific membranes is useful for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. Wavelength Maxima: Excitation 510nm, Emission 626nm
Product Details
| Alternative Name: | N-(3-Triethylammoniumpropyl)-4-(4-(dibutylamino)styryl)pyridinium dibromide |
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| Formula: | C30H49Br2N3 |
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| MW: | 611.5 |
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| CAS: | 149838-22-2 |
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| Purity: | ≥90% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells: D.R. Williams, et al.; Nat. Protoc.
3, 835 (2008),
Abstract;
FM 1-43 labeling of synaptic vesicle pools at the Drosophila neuromuscular junction: P. Verstreken, et al.; Methods Mol. Biol.
440, 349 (2008),
Abstract;
