Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Aqua431dUTP can also be used for multicolor fluorescence labeling.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
Alternative Name:
DEAC dUTP
Quantity:
Sufficient for approximately 98 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
Formulation:
Lyophilized.
Excitation maximum:
431 nm
Emission maximum:
480 nm
Extinction Coefficient:
50,000 M-1 cm-1 (431 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
Correction Factor (260 nm):
0.14
Correction Factor (280 nm):
0.14
Purity:
≥93% (HPLC)
Purity Detail:
Purified by ion-exchange chromatography.
Appearance:
Yellow solid.
Applications:
FISH
Shipping:
Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
