For ready-to-use, aqueous format, see
ENZ-42843.
Gold 525 [5(6)-Carboxyrhodamine 6G] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Gold 525 dUTP can also be used for multicolor fluorescence labeling.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
| Alternative Name: | 5(6)-Carboxyrhodamine 6G dUTP |
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| Quantity: | Sufficient for approximately 98 reactions, following the recommended protocol of Prod. No. ENZ-GEN111. |
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| Formulation: | Lyophilized. |
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| Excitation maximum: | 525 nm |
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| Emission maximum: | 551 nm |
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| Extinction Coefficient: | 92,000 M-1 cm-1 (525 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) |
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| Correction Factor (260 nm): | 0.24 |
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| Correction Factor (280 nm): | 0.29 |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Appearance: | Magenta solid. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
An evaluation and recommendation of the optimal methodologies to detect RET gene rearrangements in papillary thyroid carcinoma: T. Zhang, et al.; Genes Chromosomes Cancer
54, 168 (2015),
Abstract;
FGFR2 amplification has prognostic significance in gastric cancer: results from a large international multicentre study: X. Su, et al.; Br. J. Cancer
110, 967 (2014),
Abstract;
Full Text
Subtyping of renal cortical neoplasms in fine needle aspiration biopsies using a decision tree based on genomic alterations detected by fluorescence in situ hybridization: B. Gowrishankar, et al.; BJU Int.
114, 881 (2014),
Abstract;
Full Text
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