The JC Virus BIO-PROBE® labeled probe is prepared by nick translation of a clone of the entire JC virus genome. JC virus, a member of the papovavirus family, usually causes subclinical infections. However, in immunocompromised individuals it can cause a fatal demyelinating brain disease, progressive multifocal leukoencephalopathy. JC virus is also neurooncogenic in primates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
Product Details
Concentration:
20µg/ml
Formulation:
Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
Applications:
Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
Application Notes:
The JC Virus BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at a concentration of approximately 50ng/ml. The probe can also be used for in situ hybridization applications at concentrations of approximately 0.5 to 5μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes.The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes. Complete kits and protocols are available for in situ hybridization and detection procedures (compatible with Enzo’s POLYVIEW® PLUS and SAVIEW® PLUS detection systems for IHC).
Shipping:
Shipped on Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for one year after receipt when stored as recommended.
Technical Info/Product Notes:
BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Effect of JCV on Expression of -Catenin in Colorectal Carcinoma: I.F. Aldrubi, et al. ; Int. J. Curr. Microbiol. App. Sci 4, 215 (2015), Application(s): Detection of JCV in tissue samples, Full Text
The Validity of Real Time PCR and in Situ Hybridization in the Detection of JCV in Colonic Biopsies of Patient with Colorectal Cancer : I.F. Aldrubi, et al. ; Int. J. Curr. Microbiol. App. Sci 4, 107 (2015), Application(s): In situ hybridization (ISH), Full Text
Hexadecyloxypropyl-cidofovir (CMX001) suppresses JC virus replication in human fetal brain SVG cell cultures: Z.G. Jiang, et al.; Antimicrob. Agents Chemother. 54, 4723 (2010), Abstract; Full Text
Characterization and application of polyclonal antibodies that specifically recognize JC virus large T antigen: Y. Sunden, et al.; Acta Neuropathol. 111, 379 (2006), Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 353 (1987), Abstract;
Progressive multifocal leukoencephalopathy: JC virus detection by in situ hybridization compared with immunohistochemistry: A.J. Aksamit, et al.; Neurology 36, 499 (1986), Abstract;
Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe: A.J. Aksamit, et al.; Ann. Neurol. 18, 490 (1985), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;
