Recombinant human NGAL in monomer and dimer forms.
UniProt ID:
P80188
Species reactivity:
Human
Applications:
ELISA, IHC, WB
Application Notes:
Detects a band of ~25kDa by Western blot.
Purity Detail:
Protein A/G-affinity purified.
Formulation:
Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide.
Shipping:
Blue Ice Not Frozen
Short Term Storage:
+4°C
Scientific Background:
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) belongs to the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, and occurs as 25-kDa glycosylated single protein chain monomers which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. The expression and release of NGAL from renal tubules is dramatically increased by ischemic or nephrotoxic injury.
Product Literature References
Presence of NGAL/MMP-9 complexes in human abdominal aortic aneurysms: M. Folkesson, et al.; Thromb. Haemost. 98, 427 (2007), Abstract;
Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils: L. Kjeldsen, et al.; J. Immunol. Methods 198, 155 (1996), Abstract;
General Literature References
Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), Abstract;
Ultra sensitive (1.2 pg/ml) ELISA kit, enabling reduced input sample and matrix interference, for the quantification of KIM-1, an early biomarker for kidney injury or disease.
