Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate DEVD-pNA.
Product Specification
| Alternative Name: | CPP32, Yama, Apopain |
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| Applications: | Colorimetric detection
Activity assay
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| Handling: | Store Lysis Buffer, Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl DEVD-pNA (4mM), 100µl DTT (1M), 25ml Dilution Buffer. |
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| Technical Info/Product Notes: | The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P42574 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Ruxolitinib combined with vorinostat suppresses tumor growth and alters metabolic phenotype in hematological diseases: M. Civallero, et al.; Oncotarget
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EZH2 is overexpressed in adrenocortical carcinoma and is associated with disease progression: C. Drelon, et al.; Hum. Mol. Genet.
25, 2789 (2016),
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Full Text
Activity of BKM120 and BEZ235 against Lymphoma Cells: M. Civallero, et al.; Biomed Res. Int.
2015, Article ID 870918 (2015),
Application(s): Measurement of caspase-3 activity in human lymphoma cell lines,
Abstract;
Full Text
Direct antiapoptotic effects of growth hormone are mediated by PI3K/Akt pathway in the chicken bursa of Fabricius: J.L. Luna-Acosta, et al.; Gen. Comp. Endocrinol.
224, 148 (2015),
Application(s): Caspase-3 assay in chicken B cell lysate,
Abstract;
Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells: C.C. Chen, et al.; Drug Des. Devel. Ther.
8, 459 (2014),
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Full Text
Liquiritigenin induces tumor cell death through mitogen-activated protein kinase- (MPAKs-) mediated pathway in hepatocellular carcinoma cells: D. Wang, et al.; Biomed. Res. Int.
2014, Article ID 965316 (2014),
Application(s): Measurement of caspase-3 activity in PLC/PRF/5 (p53 mutant) and HepG2 (p53 wild type) human tumor cells,
Abstract;
Full Text
A2B adenosine receptor blockade inhibits growth of prostate cancer cells: Q. Wei, et al.; Purinergic Signal.
9, 271 (2013),
Application(s): Measurement of caspase-3 activity in human PC-3 cells,
Abstract;
Therapeutic benefits of the group B3 vitamin nicotinamide in mice with lethal endotoxemia and polymicrobial sepsis: H. Yuan, et al.; Pharmacol. Res.
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Abstract;
Activation of the P2Y1 receptor induces apoptosis and inhibits proliferation of prostate cancer cells: Q. Wei, et al. ; Biochem. Pharmacol.
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Application(s): Measurement of caspase-3 activity in human PC-3 cells,
Abstract;
Seasonal study of apoptotic markers in lizard oviduct: L. Assisi, et al.; J. Exp. Zool. A Ecol. Genet. Physiol.
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