Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2
Clone:
1B6
Host:
Mouse
Isotype:
IgG1
Immunogen:
Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
UniProt ID:
Q9BYX4
Source:
Purified from hybridoma tissue culture supernatant.
Species reactivity:
Human, Mouse
Applications:
ELISA, WB
Recommended Dilutions/Conditions:
Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:
Protein G-affinity purified.
Formulation:
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow easier access of CAD, thereby accelerating the degradation of genomic DNA.
Regulatory Status:
RUO - Research Use Only
Western blot analysis of MDA5 monoclonal antibody (1B6) (Prod. No. ALX-804-878): Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.
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General Literature References
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature 441, 101 (2006), Abstract;
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002), Abstract;
