Product Details
Alternative Name: | Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2 |
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Host: | Rabbit |
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Immunogen: | Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555). |
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UniProt ID: | Q9BYX4 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human
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Applications: | IP, WB
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Recommended Dilutions/Conditions: | Immunoprecipitation (1:100) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Epitope-affinity purified. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells: X. Yin, et al.; Cell Rep.
32, 108628 (2021),
Application(s): Western Blot,
Abstract;
Full Text
DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection: S. Pattabhi, et al.; J. Interferon Cytokine Res.
39, 331 (2019),
Abstract;
The 14-3-3η chaperone protein promotes antiviral innate immunity via facilitating MDA5 oligomerization and intracellular redistribution: J.P. Lin, et al.; PLoS Pathog.
15, e1007582 (2019),
Abstract;
Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein: H.P. Chiu, et al.; PLoS Pathog.
14, e1007166 (2018),
Abstract;
Full Text
Disruption of MDA5-Mediated Innate Immune Responses by the 3C Proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68: Y. Rui, et al.; J. Virol.
91, e00546-17 (2017),
Abstract;
Full Text
Hepatitis E virus persists in the presence of a type III interferon response: X. Yin, et al.; PLoS Pathog.
13, e1006417 (2017),
Abstract;
Full Text
Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia: T.L. Cuellar, et al.; J. Cell Biol.
216, 3535 (2017),
Abstract;
Full Text
Association of a Network of Interferon-Stimulated Genes with a Locus Encoding a Negative Regulator of Non-conventional IKK Kinases and IFNB1: S. Jeidane, et al.; Cell Rep.
17, 425 (2016),
Application(s): Western Immunoblot Analyses,
Abstract;
Full Text
ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA: C. Lässig, et al.; eLife
4, e10859 (2015),
Application(s): Sense RNA from a broad range of viruses, dependent signaling,
Abstract;
Full Text
Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils: N. Tamassia, et al.; J. Immunol.
181, 6563 (2008),
Abstract;
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol.
82, 335 (2008),
Abstract;
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ.
15, 1804 (2008),
Abstract;
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS
99, 637 (2002),
Abstract;
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