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MDA5 (human) polyclonal antibody (AT113)



ALX-210-935-C100	100 µg	457.00 USD
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Product Details

Alternative Name:	Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2

Host:	Rabbit

Immunogen:	Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).

UniProt ID:	Q9BYX4

Source:	Purified from rabbit serum.

Species reactivity:	Human

Applications:	IP, WB

Recommended Dilutions/Conditions:	Immunoprecipitation (1:100) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Epitope-affinity purified.

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Use/Stability:	Stable for at least 1 year after receipt when stored as recommended.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Scientific Background:	MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.

Regulatory Status:	RUO - Research Use Only

Product Literature References

MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells: X. Yin, et al.; Cell Rep. 32, 108628 (2021), Application(s): Western Blot, Abstract; Full Text

DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection: S. Pattabhi, et al.; J. Interferon Cytokine Res. 39, 331 (2019), Abstract;

The 14-3-3η chaperone protein promotes antiviral innate immunity via facilitating MDA5 oligomerization and intracellular redistribution: J.P. Lin, et al.; PLoS Pathog. 15, e1007582 (2019), Abstract;

Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein: H.P. Chiu, et al.; PLoS Pathog. 14, e1007166 (2018), Abstract; Full Text

Disruption of MDA5-Mediated Innate Immune Responses by the 3C Proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68: Y. Rui, et al.; J. Virol. 91, e00546-17 (2017), Abstract; Full Text

Hepatitis E virus persists in the presence of a type III interferon response: X. Yin, et al.; PLoS Pathog. 13, e1006417 (2017), Abstract; Full Text

Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia: T.L. Cuellar, et al.; J. Cell Biol. 216, 3535 (2017), Abstract; Full Text

Association of a Network of Interferon-Stimulated Genes with a Locus Encoding a Negative Regulator of Non-conventional IKK Kinases and IFNB1: S. Jeidane, et al.; Cell Rep. 17, 425 (2016), Application(s): Western Immunoblot Analyses, Abstract; Full Text

ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA: C. Lässig, et al.; eLife 4, e10859 (2015), Application(s): Sense RNA from a broad range of viruses, dependent signaling, Abstract; Full Text

Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils: N. Tamassia, et al.; J. Immunol. 181, 6563 (2008), Abstract;

Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008), Abstract;

The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract;

General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002), Abstract;

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