Product Details
Alternative Name: | Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2 |
|
Clone: | Hely-1 |
|
Host: | Mouse |
|
Isotype: | IgG1 |
|
Immunogen: | Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555). |
|
UniProt ID: | Q9BYX4 |
|
Source: | Purified from concentrated hybridoma tissue cell culture supernatant. |
|
Species reactivity: | Human
|
|
Applications: | ELISA, IP, WB
|
|
Recommended Dilutions/Conditions: | Immunoprecipitation (1:100) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Application Notes: | ELISA Immunoprecipitation (1:100) Western Blot (1:1'000) |
|
Purity Detail: | Protein G-affinity purified. |
|
Formulation: | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
|
Handling: | Avoid freeze/thaw cycles. |
|
Shipping: | Shipped on Blue Ice |
|
Short Term Storage: | +4°C |
|
Long Term Storage: | -20°C |
|
Scientific Background: | MDA5 and RIG-I are highly conserved helicases involved in the innate immune response to virus. MDA-5 ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. After binding nonself-ligand, RIG-I and MDA5 interact via their CARD domains with the signaling-adaptor molecule, IPS-1 (also known as MAVS, VISA and Cardif), which leads to the induced expression of IFN-β, IRF3-target genes and NF- κB target genes to drive antiviral and inflammatory responses. |
|
Regulatory Status: | RUO - Research Use Only |
|
General Literature References
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature
441, 101 (2006),
Abstract;
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS
99, 637 (2002),
Abstract;
Related Products