Product Details
| Alternative Name: | APO-1L, CD178, CD95L, TNFSF 6 |
| |
| Clone: | 2C101 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Recombinant human FasL (CD95L; APO-1L; CD178) (extracellular domain). |
| |
| UniProt ID: | P48023 |
| |
| Species reactivity: | Human
|
| |
| Applications: | Flow Cytometry, ICC, FUNC
|
| |
| Recommended Dilutions/Conditions: | Suggested starting dilution is >1:100. , Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Excellent for Functional Application (neutralizing). |
| |
| Purity Detail: | Affinity purified. |
| |
| Formulation: | Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA. Contains no preservatives. |
| |
| Use/Stability: | Stable for at least 6 months when stored at +4°C. |
| |
| Handling: | Keep sterile. Do not freeze. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | +4°C |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Figure: Schematic structure of recombinant human FasL (extracellular domain).
Figure: Flow cytometric detection of FasL expression in stimulated H9 T-cells.Method: H9 T-cells (1x106) were either left untreated (open histogram) or stimulated with 10ng/ml PMA and 1µg/ml ionomycin (filled histogram). After 12 hours cells were harvested and stained with anti-FasL 2C101 (1µg/ml), followed by biotinylated goat anti-mouse IgG and streptavidin-PE.
Figure: Inhibition of apoptosis in anti-CD3 stimulated Jurkat T-cells by anti-Fas L.Method: Jurkat JR cells (2x106) were stimulated with plate-bound anti-CD3 antibodies (10µg/ml) in the presence or absence of the indicated concentrations of anti-FasL 2C101 or a control IgG1 mouse monoclonal antibody. After 48 hours apoptosis was evaluated by flow cytometric analysis of PI-stained nuclei.
Figures: Anti-FasL MAb
ALX-804-232 on HEK 293 wt. and HEK 293-FasL clone cells expressing FasL fusion protein. HEK 293 cells transfected with membrane bound FasL (right figure) were compared with HEK 293 untransfected control cells (left figure). "
Figure: Comparison of anti-FasL MAbs 804-231 and 804-232 on 293-FasL clone cells expressing FasL fusion protein. Comparable results were obtained for all other MAbs to hFasL recommended for flow cytometry.
Please mouse over
Product Literature References
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt et al.; Eur. J. Immunol.
30, 1769 (2000),
Abstract;
