E-cadherin monoclonal antibody (ECCD-2)



ALX-804-202-C100	100 µg	764.00 USD
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Product Details

Alternative Name:	Uvomorulin, CD324, Epithelial cadherin, Cadherin-1

Clone:	ECCD-2

Host:	Rat

Isotype:	IgG2a

Immunogen:	Mouse liver E-cadherin fragment.

UniProt ID:	P09803

Source:	Obtained by fusing the P3-X63-Ag8 myeloma cell line with splenocytes of Donryu rat immunized with mouse liver E-cadherin fragment.

Species reactivity:	Human, Mouse Dog

Crossreactivity:	Does not cross-react with bovine or chicken E-cadherin.

Applications:	IHC (FS), WB, FUNC

Recommended Dilutions/Conditions:	Immunohistochemistry (frozen sections, 10µg/ml) Western Blot (10µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Not recommended for Immunohistochemistry (paraffin sections). Functional Application: Does not inhibit E-cadherin dependent cell-cell contact and can be used to analyze the mechanism.

Purity Detail:	Purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography.

Formulation:	Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA. Contains no preservatives.

Reconstitution:	Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl₂, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.

Use/Stability:	Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 6 months when stored at +4°C and for 1 year when stored at -20°C.

Handling:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Shipping:	Shipped on Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Technical Info/Product Notes:	Buffers should contain Ca²⁺ to stabilize cadherin antigen. In case of immunohistochemical staining of acetone fixed frozen sections, Ca²⁺ is not necessary.

Regulatory Status:	RUO - Research Use Only

Product Literature References

Inverse correlation between the extent of N-glycan branching and intercellular adhesion in epithelia. Contribution of the Na,K-ATPase beta1 subunit: O. Vagin, et al.; J. Biol. Chem. 283, 2192 (2008), Abstract; Full Text

Recruitment and activation of Rac1 by the formation of E-cadherin- mediated cell-cell adhesion sites: M. Nakagawa, et al.; J. Cell. Sci. 114, 1829 (2001), Abstract;

The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells: S. Wallis, et al.; Mol. Biol. Cell. 11, 1077 (2000), Abstract;

Role of IQGAP1, a target of the small GTPase Cdc42 and Rac1, in regulation of E-cadherin-mediated cell-cell adhesion: S. Kuroda, et al.; Science 281, 832 (1998), Abstract;

Recognition of E-cadherin on epithelial cells by the mucosal T cell integrin alpha M290 beta 7 (alpha E beta 7): P.I. Karecla, et al.; Eur. J. Immunol. 25, 852 (1995), Abstract;

Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science 251, 1451 (1991), Abstract;

The cadherins: cell-cell adhesion molecules controlling animal morphogenesis: M. Takeichi; Development 102, 639 (1988), Abstract;

Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA: A. Nagafuchi, et al.; Nature 329, 341 (1987), Abstract;

N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin: Y. Shirayoshi, et al.; Cell Struct. Funct. 11, 245 (1986), Abstract;