Replaces Prod. #: BML-SA180
Product Details
| Alternative Name: | VDR, 1,25-Dihydroxyvitamin D3 receptor |
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| Clone: | 9A7 |
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| Host: | Rat |
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| Isotype: | IgG2b |
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| Immunogen: | Partially purified chicken intestinal cytoplasmic VDR (vitamin D3 receptor). |
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| UniProt ID: | O42392 |
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| Species reactivity: | Human, Mouse, Rat
Amphibian, Chicken, Fish, Hamster
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| Specificity: | Recognizes the occupied and unoccupied forms of VDR. Recognizes an epitope between aa 89 and 105 of human VDR. |
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| Crossreactivity: | Does not cross-react with ER (estrogen receptor) or GR (glucocorticoid receptor). |
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| Applications: | ICC, IP, WB
ChIP, Gel Supershift
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | In avians detects bands of ~58kDa and ~60kDa by Western blot, while in mammalian species the VDRs appear as a single band within the ~52-55kDa range. |
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| Formulation: | Liquid. Purified antibody containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Western blot analysis of VDR in human skin fibroblast extract using Vitamin D3 receptor, mAb (9A7) (Prod. No.
ALX-804-021)."
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Product Literature References
Sentrin/SUMO specific proteases as novel tissue-selective modulators of vitamin D receptor-mediated signaling: W.P. Lee, et al.; PLoS One
9, e89506 (2014),
Application(s): WB using human cell lysate,
Abstract;
Identification of amino acid sequence in the hinge region of human vitamin D receptor that transfers a cytosolic protein to the nucleus: T. Michigami, et al.; J. Biol. Chem.
274, 33531 (1999),
Abstract;
Prediction of bone density from vitamin D receptor alleles: N.A. Morrison, et al.; Nature
367, 284 (1994),
Abstract;
Baculovirus-mediated expression of the human vitamin D receptor. Functional characterization, vitamin D response element interactions, and evidence for a receptor auxiliary factor: P.N. MacDonald, et al.; J. Biol. Chem.
266, 18808 (1991),
Abstract;
Full Text
Immunohistochemical detection of 1,25-dihydroxyvitamin D3 receptors and estrogen receptors by monoclonal antibodies: comparison of four immunoperoxidase methods: P. Milde, et al.; J. Histochem. Cytochem.
37, 1609 (1989),
Abstract;
Immunocytochemical localization of the 1,25-dihydroxyvitamin D3 receptor in target cells: T.L. Clemens, et al.; Endocrinology
122, 1224 (1988),
Abstract;
Molecular biology of the vitamin D hormone: M.R. Haussler, et al.; Recent Progr. Horm. Res.
44, 263 (1988),
Abstract;
Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein: J.W. Pike, et al.; J. Biol. Chem.
258, 1289 (1983),
Abstract;
Full Text
Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor: J.W. Pike, et al.; PNAS
79, 7719 (1982),
Abstract;
