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Vitamin D3 receptor monoclonal antibody (9A7)

ALX-804-021-C100 100 µg 778.00 USD
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Replaces Prod. #: BML-SA180

Product Details

Alternative Name:VDR, 1,25-Dihydroxyvitamin D3 receptor
Immunogen:Partially purified chicken intestinal cytoplasmic VDR (vitamin D3 receptor).
UniProt ID:O42392
Species reactivity:Human, Mouse, Rat
Amphibian, Chicken, Fish, Hamster
Specificity:Recognizes the occupied and unoccupied forms of VDR. Recognizes an epitope between aa 89 and 105 of human VDR.
Crossreactivity:Does not cross-react with ER (estrogen receptor) or GR (glucocorticoid receptor).
Applications:ICC, IP, WB
ChIP, Gel Supershift
Recommended Dilutions/Conditions:Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:In avians detects bands of ~58kDa and ~60kDa by Western blot, while in mammalian species the VDRs appear as a single band within the ~52-55kDa range.
Formulation:Liquid. Purified antibody containing 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
Vitamin D3 receptor monoclonal antibody (9A7) Western blot
Figure: Western blot analysis of VDR in human skin fibroblast extract using Vitamin D3 receptor, mAb (9A7) (Prod. No. ALX-804-021)."
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Vitamin D3 receptor monoclonal antibody (9A7) Western blot

Product Literature References

Sentrin/SUMO specific proteases as novel tissue-selective modulators of vitamin D receptor-mediated signaling: W.P. Lee, et al.; PLoS One 9, e89506 (2014), Application(s): WB using human cell lysate, Abstract;
Identification of amino acid sequence in the hinge region of human vitamin D receptor that transfers a cytosolic protein to the nucleus: T. Michigami, et al.; J. Biol. Chem. 274, 33531 (1999), Abstract;
Prediction of bone density from vitamin D receptor alleles: N.A. Morrison, et al.; Nature 367, 284 (1994), Abstract;
Baculovirus-mediated expression of the human vitamin D receptor. Functional characterization, vitamin D response element interactions, and evidence for a receptor auxiliary factor: P.N. MacDonald, et al.; J. Biol. Chem. 266, 18808 (1991), Abstract; Full Text
Immunohistochemical detection of 1,25-dihydroxyvitamin D3 receptors and estrogen receptors by monoclonal antibodies: comparison of four immunoperoxidase methods: P. Milde, et al.; J. Histochem. Cytochem. 37, 1609 (1989), Abstract;
Immunocytochemical localization of the 1,25-dihydroxyvitamin D3 receptor in target cells: T.L. Clemens, et al.; Endocrinology 122, 1224 (1988), Abstract;
Molecular biology of the vitamin D hormone: M.R. Haussler, et al.; Recent Progr. Horm. Res. 44, 263 (1988), Abstract;
Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein: J.W. Pike, et al.; J. Biol. Chem. 258, 1289 (1983), Abstract; Full Text
Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor: J.W. Pike, et al.; PNAS 79, 7719 (1982), Abstract;

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