Alternative Name: | CD27L, TNFSF 7 |
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MW: | ~28kDa (SDS-PAGE). |
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Source: | Produced in HEK 293 cells. The extracellular domain of mouse CD70 (CD27L) (aa 52-195) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag. |
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UniProt ID: | O55237 |
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Gene/Protein Identifier: | A000547 (UCSD Signaling Gateway ID) |
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Concentration: | 0.1mg/ml after reconstitution. |
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Formulation: | Lyophilized. Contains PBS. |
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Purity: | ≥95% (SDS-PAGE) |
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Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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Species reactivity: | Human, Mouse
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Specificity: | Binds to human and mouse CD27. |
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Applications: | ELISA
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Application Notes: | ELISA: binds to CD27 receptor. Can be used together with anti-FLAG® MAb to detect membrane-bound human and mouse CD27. rmsCD70 saturates its receptor at a concentration range of 0.06-60ng/ml. |
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Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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Scientific Background: | The CD27 ligand (CD27L, CD70) is a member of the tumor necrosis factor (TNF) family. CD27L is a type II, transmembrane protein. Engagement of CD27 by CD27L induces recruitment of TNF receptor-associated factor 2 (TRAF2) and TRAF3. CD27-CD70 interactions have been reported to be involved in several different immune mechanisms such as T, NK and B lymphocytes interactions and costimulation, early thymic differentiation, autoimmune and viral diseases and apoptosis. CD70 is a marker for mature B cells. |
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Regulatory Status: | RUO - Research Use Only |
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