| Alternative Name: | TR2, ATAR, LIGHT-R, TNFRSF 14, Herpes virus entry mediator A |
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| MW: | ~58kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human HVEM (aa 39-200) is fused to the Fc portion of human IgG1. |
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| UniProt ID: | Q92956 |
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| Concentration: | 1mg/ml, after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds human and mouse LIGHT. |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to LIGHT).
Detection of membrane-bound human and mouse LIGHT (HVEML) in combination with PAb to human IgG1. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | HVEM (TR2, ATAR, HVEA) is a 36 kDa type I transmembrane protein belonging to the TNF receptor family. HVEM contains 3 cysteine-rich repeats in the extracellular portion which is typical of TNFR family members. HVEM mediates Herpes Simplex Virus-1 infection of T cells. Ligation of HVEM by its cellular trimeric ligand LIGHT (Prod. No. ALX-522-018) induces recruitment to the cytoplasmic domain of the signaling molecules TRAF2 and 5. Activation in vitro of HVEM leads to induction of nuclear factors NFκB and AP-1. The outcome of HVEM activation on T cells is still controversial. A second ligand for HVEM is soluble LTalpha. Historical data has shown the inhibition of LIGHT-mediated effects, such as the cytotoxic effect on HT29 target cells. Prevents Herpes Simplex Virus-1 infection of T-cells. |
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| Regulatory Status: | RUO - Research Use Only |
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