A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results.
Prod. No. ALX-211-056 is recommended for use with the following product:
ALX-804-340 MAb to DLC1 (10D6)
Product Details
| Host: | Goat |
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| Immunogen: | Rat IgM. |
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| Species reactivity: | Rat
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| Specificity: | Recognizes the heavy chain of rat IgM. |
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| Applications: | Flow Cytometry
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| Recommended Dilutions/Conditions: | Flow Cytometry (>1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Purified by affinity chromatography on rat IgM covalently linked to Sepharose™-4B. Cross-adsorbed with rat IgG. Cross-reactivity with IgM from other species has not been evaluated. |
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| Formulation: | Liquid. In PBS, pH 7.4, containing 0.1% sodium azide. |
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| Handling: | Protect from light. Do not freeze. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | SEPHAROSE is a trademark of GE Healthcare companies. |
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| Regulatory Status: | RUO - Research Use Only |
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