PARP-1 (BRCT Domain) polyclonal antibody

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ALX-210-540-R100	100 µl	279.00 USD
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Alternative Name:	Poly(ADP-ribose) polymerase-1

Host:	Rabbit

Immunogen:	Recombinant human PARP-1 (BRCT domain) (aa 385-524) fused to a His-tag (Prod. No. ALX-201-255).

UniProt ID:	P09874

Species reactivity:	Human, Mouse

Applications:	IP, WB

Recommended Dilutions/Conditions:	Immunoprecipitation (1:200) Western Blot (1:8,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Does not work for ICC.

Purity Detail:	Ammonium sulfate precipitate.

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Use/Stability:	Stable for at least one year when stored at +4°C.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	+4°C

Regulatory Status:	RUO - Research Use Only

PARP-1 (BRCT Domain) polyclonal antibody Western blot

Figure 1: Western blot analysis of endogenous and recombinant PARP-1 using PAb to PARP-1 (BRCT Domain) (Prod. No. ALX-210-540). Lane 1: Recombinant human His-tagged PARP-1 BRCT domain (Prod. No. ALX-201-255) (30ng). Lane 2: Hela 5x10⁴ cells. Lane 3: MEFs 10⁵ cells. Lane 4: HL60 10⁵ cells. Lane 5: HL60 10⁵cells treated with etoposide for 4 hours. The antibody detects the 85kDa cleavage product."

PARP-1 (BRCT Domain) polyclonal antibody Immunoprecipitation

Figure 2: Immunoprecipitation of endogenous PARP-1 from HeLa cells using PAb to PARP-1 (BRCT Domain) (Prod. No. ALX-210-540) (1:200). Lane 1: PAb to PARP-1 (BRCT Domain). Lane 2: Pre-immuneserum."

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PARP-1 (BRCT domain) (human), (recombinant) (His-tag)

Produced in E. coli. Human PARP-1 (BRCT domain) (aa 385-524) is fused to a His-tag., ≥99% (SDS-PAGE) | Print as PDF

ALX-201-255-C020

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