Product Details
Alternative Name: | PBEF1, Pre-B-cell colony enhancing factor 1, Nicotinamide phosphoribosyltransferase |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to aa 40-56 (R40EKKTENSKLRKVKYEE56) of human Nampt (Visfatin/PBEF). |
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UniProt ID: | P43490 |
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Species reactivity: | Human
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Crossreactivity: | Weakly cross-reacts with mouse Nampt. |
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Applications: | ELISA, WB
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Recommended Dilutions/Conditions: | ELISA (direct and indirect: 1:2,000-1:5,000) Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Liquid. In PBS, pH 7.4. Contains no preservatives. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Western blot analysis of Nampt (human) (rec.) (His) (Prod. No.
ALX-201-319) using PAb to Nampt (human) (NT) (Prod. No.
ALX-210-426) at 1:2’000 dilution."
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Product Literature References
Visfatin, an adipocytokine with proinflammatory and immunomodulating properties: A.R. Moschen, et al.; J. Immunol.
178, 1748 (2007),
Abstract;
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